Artículo
Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges
Autor/es | Olmedo Vicente, Erika
Désamoré, Aurélie Simón Porcar, Violeta Slotte, Tanja Arroyo Marín, Juan |
Departamento | Universidad de Sevilla. Departamento de Biología Vegetal y Ecología |
Fecha de publicación | 2023 |
Fecha de depósito | 2023-09-29 |
Publicado en |
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Resumen | Background: Microsatellite markers were developed for distylous Linum suffruticosum and tested in the monomorphic sister species Linum tenuifolium. These species are perennial herbs endemic to the western and northwestern ... Background: Microsatellite markers were developed for distylous Linum suffruticosum and tested in the monomorphic sister species Linum tenuifolium. These species are perennial herbs endemic to the western and northwestern Mediterranean, respectively, with a partially overlapping distribution area. Methods and results: We developed 12 microsatellite markers for L. suffruticosum using next generation sequencing, and assessed their polymorphism and genetic diversity in 152 individuals from seven natural populations. The markers displayed high polymorphism, with two to 16 alleles per locus and population, and average observed and expected heterozygosities of 0.833 and 0.692, respectively. All loci amplified successfully in the sister species L. tenuifolium, and 150 individuals from seven populations were also screened. The polymorphism exhibited was high, with two to ten alleles per locus and population, and average observed and expected heterozygosities of 0.77 and 0.62, respectively. Conclusions: The microsatellite markers identified in L. suffruticosum and tested in L. tenuifolium are a powerful tool to facilitate future investigations of the population genetics, mating patterns and hybridization between both Linum species in their contact zone. |
Agencias financiadoras | European Research Council (ERC) Ministerio de Ciencia e Innovación (MICIN). España European Union (UE) |
Identificador del proyecto | 757451
CGL2013-45037-P PGC2018 099608 B 100 PID2021-122715NB-I00 897890 |
Cita | Olmedo Vicente, E., Désamoré, A., Simón Porcar, V., Slotte, T. y Arroyo Marín, J. (2023). Development of microsatellite markers for sister species Linum suffruticosum and Linum tenuifolium in their overlapping ranges. Molecular Biology Reports, 50 (9), 7927-7933. https://doi.org/10.1007/s11033-023-08471-9. |
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