dc.creator | Lara Moreno, Alba | es |
dc.creator | Merchán Ignacio, Francisco | es |
dc.creator | Morillo, Esmeralda | es |
dc.creator | Zampolli, Jessica | es |
dc.creator | Di Gennaro, Patrizia | es |
dc.creator | Villaverde, Jaime | es |
dc.date.accessioned | 2023-06-02T09:50:59Z | |
dc.date.available | 2023-06-02T09:50:59Z | |
dc.date.issued | 2023 | |
dc.identifier.citation | Lara Moreno, A., Merchán Ignacio, F., Morillo, E., Zampolli, J., Di Gennaro, P. y Villaverde, J. (2023). Genome analysis for the identification of genes involved in phenanthrene biodegradation pathway in Stenotrophomonas indicatrix CPHE1. Phenanthrene mineralization in soils assisted by integrated approaches.. Frontiers in Bioengineering and Biotechnology, 11, 1158177. https://doi.org/10.3389/fbioe.2023.1158177. | |
dc.identifier.issn | 2296-4185 | es |
dc.identifier.uri | https://hdl.handle.net/11441/146877 | |
dc.description.abstract | Phenanthrene (PHE) is a highly toxic compound, widely present in soils. For this
reason, it is essential to remove PHE from the environment. Stenotrophomonas
indicatrix CPHE1 was isolated from an industrial soil contaminated by polycyclic
aromatic hydrocarbons (PAHs) and was sequenced to identify the PHE degrading
genes. Dioxygenase, monooxygenase, and dehydrogenase gene products annotated
in S. indicatrix CPHE1 genome were clustered into different trees with reference
proteins. Moreover, S. indicatrix CPHE1 whole-genome sequences were compared to
genes of PAHs-degrading bacteria retrieved from databases and literature. On these
basis, reverse transcriptase-polymerase chain reaction (RT-PCR) analysis pointed out
that cysteine dioxygenase (cysDO), biphenyl-2,3-diol 1,2-dioxygenase (bphC), and
aldolase hydratase (phdG) were expressed only in the presence of PHE. Therefore,
different techniques have been designed to improve the PHE mineralization process
in five PHE artificially contaminated soils (50 mg kg−1
), including biostimulation, adding
a nutrient solution (NS), bioaugmentation, inoculating S. indicatrix CPHE1 which was
selected for its PHE-degrading genes, and the use of 2-hydroxypropyl-β-cyclodextrin
(HPBCD) as a bioavailability enhancer. High percentages of PHE mineralization were
achieved for the studied soils. Depending on the soil, different treatments resulted to
be successful; in the case of a clay loam soil, the best strategy was the inoculation of S.
indicatrix CPHE1 and NS (59.9% mineralized after 120 days). In sandy soils (CR and R
soils) the highest percentage of mineralization was achieved in presence of HPBCD
and NS (87.3% and 61.3%, respectively). However, the combination of CPHE1 strain,
HPBCD, and NS showed to be the most efficient strategy for sandy and sandy loam
soils (LL and ALC soils showed 35% and 74.6%, respectively). The results indicated a
high degree of correlation between gene expression and the rates of mineralization. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad y Agencia Estatal de Investigación de España, y fondos europeos FEDER (AEI/FEDER, UE) - CMT 2017-82472- C2-1-R | es |
dc.description.sponsorship | Consejo de Transformación Económica, Industria, Conocimiento y Universidad de la Junta de Andalucía. España - FEDER Andalucía PO 2014–2020/PY20_01069 | es |
dc.format | application/pdf | es |
dc.format.extent | 1158177 | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Bioengineering and Biotechnology, 11, 1158177. | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | phenanthrene | es |
dc.subject | Stenotrophomonas indicatrix | es |
dc.subject | CPHE1 | es |
dc.subject | mineralization | es |
dc.subject | phenanthrene biodegradation pathway | es |
dc.subject | genes expression | es |
dc.title | Genome analysis for the identification of genes involved in phenanthrene biodegradation pathway in Stenotrophomonas indicatrix CPHE1. Phenanthrene mineralization in soils assisted by integrated approaches. | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología y Parasitología | es |
dc.relation.projectID | CMT 2017-82472- C2-1-R | es |
dc.relation.projectID | PY20_01069 | es |
dc.relation.publisherversion | https://dx.doi.org/10.3389/fbioe.2023.1158177 | es |
dc.identifier.doi | 10.3389/fbioe.2023.1158177 | es |
dc.journaltitle | Frontiers in Bioengineering and Biotechnology | es |
dc.publication.volumen | 11 | es |
dc.publication.initialPage | 1158177 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.contributor.funder | Junta de Andalucía | es |