dc.creator | Giráldez Pérez, Rosa María | es |
dc.creator | Grueso Molina, Elia María | es |
dc.creator | Jiménez Aguayo, Raquel | es |
dc.creator | Carbonero, Alfonso | es |
dc.creator | González Bravo, Marina | es |
dc.creator | Kuliszewska, Edyta | es |
dc.creator | Prado Gotor, Rafael | es |
dc.date.accessioned | 2023-05-15T17:25:31Z | |
dc.date.available | 2023-05-15T17:25:31Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | Giráldez Pérez, R.M., Grueso Molina, E.M., Jiménez Aguayo, R., Carbonero, A., González Bravo, M., Kuliszewska, E. y Prado Gotor, R. (2022). Use of Nanoparticles to Prevent Resistance to Antibiotics—Synthesis and Characterization of Gold Nanosystems Based on Tetracycline. Pharmaceutics, 14 (9). https://doi.org/10.3390/pharmaceutics14091941. | |
dc.identifier.issn | 1999-4923 | es |
dc.identifier.uri | https://hdl.handle.net/11441/146040 | |
dc.description.abstract | Antimicrobial resistance (AMR) is a serious public health problem worldwide which,
according to the World Health Organization (WHO), requires research into new and more effective drugs. In this work, both gold nanoparticles covered with 16-3-16 cationic gemini surfactant
(Au@16-3-16) and DNA/tetracycline (DNA/TC) intercalated complexes were prepared to effectively
transport tetracycline (TC). Synthesis of the Au@16-3-16 precursor was carried out by using trihydrated
gold, adding sodium borohydride as a reducing agent and the gemini surfactant 16-3-16 as stabilizing
agent. Circular dichroism and atomic force microscopy techniques were then used to ascertain the
optimal R range of the relationship between the concentrations of Au@16-3-16 and the DNA/TC
complex (R = CAu@16-3-16/CDNA) that allow the obtainment of stable and compact nanosystems, these
characteristics being fundamental for their use as antibiotic transporters. Stability studies over time
were carried out for distinct selected Au@16-3-16 and Au@16-3-16/DNA-TC nanoformulations using
the ultraviolet–visible spectrophotometry technique, checking their stability for at least one month.
In addition, in order to know the charge and size distribution of the nanocomplexes, DLS and zeta
potential measurements were performed in the solution. The results showed that the characterized
nanosystems were highly charged, stable and of a reduced size (<100 nm) that allows them to cross
bacterial membranes effectively (>1 µm). Once the different physicochemical characteristics of the gold
nanosystems were measured, Au@16-3-16 and Au@16-3-16/DNA-TC were tested on Escherichia coli
and Staphylococcus aureus to study their antibacterial properties and internalization capacity in microbes. Differences in the interaction of the precursors and the compacted nanosystems generated
were observed in Gram-positive and Gram-negative bacteria, possibly due to membrane damage
or electrostatic interaction with internalization by endocytosis. In the internalization experiments,
depending on the treatment application time, the greatest bacterial destruction was observed for all
nanoformulations explored at 18 h of incubation. Importantly, the results obtained demonstrate that
both new nanosystems based on TC and Au@16-3-16 precursors have optimal antimicrobial properties
and would be beneficial for use in patients, avoiding possible side effects. | es |
dc.description.sponsorship | Junta de Andalucía FQM-386 | es |
dc.description.sponsorship | Universidad Pablo de Olavide 00001297 | es |
dc.description.sponsorship | Universidad de Sevilla 00000274 | es |
dc.format | application/pdf | es |
dc.format.extent | 30 p. | es |
dc.language.iso | eng | es |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | es |
dc.relation.ispartof | Pharmaceutics, 14 (9). | |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Antibiotic resistance | es |
dc.subject | DNA | es |
dc.subject | Gemini surfactant | es |
dc.subject | Gold nanoparticles | es |
dc.subject | Tetracycline | es |
dc.title | Use of Nanoparticles to Prevent Resistance to Antibiotics—Synthesis and Characterization of Gold Nanosystems Based on Tetracycline | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Física | es |
dc.relation.projectID | FQM-386 | es |
dc.relation.projectID | 00001297 | es |
dc.relation.projectID | 00000274 | es |
dc.relation.publisherversion | https://doi.org/10.3390/pharmaceutics14091941 | es |
dc.identifier.doi | 10.3390/pharmaceutics14091941 | es |
dc.journaltitle | Pharmaceutics | es |
dc.publication.volumen | 14 | es |
dc.publication.issue | 9 | es |
dc.contributor.funder | Junta de Andalucía | es |
dc.contributor.funder | Universidad Pablo de Olavide | es |
dc.contributor.funder | Universidad de Sevilla | es |