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Artículo
cDNA Sequence and Genomic Structure of the Rat Ret Proto-Oncogene
Autor/es | Matera, Ivana
Miguel Rodríguez, Manuel de Fernández-Santos, José María Santamaria, Giuseppe Puliti, Aldamaria Ravazzolo, Roberto Romeo, Giovanni Galera Davidson, Hugo Ceccherini, Isabella |
Departamento | Universidad de Sevilla. Departamento de Citología e Histología Normal y Patológica |
Fecha de publicación | 2000-06-01 |
Fecha de depósito | 2023-02-06 |
Publicado en |
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Resumen | The RET proto-oncogene, a member of the Receptor Tyrosine Kinase family, plays a crucial role during the development of the excretory system and the enteric nervous system, as demonstrated by in vivo animal studies and by ... The RET proto-oncogene, a member of the Receptor Tyrosine Kinase family, plays a crucial role during the development of the excretory system and the enteric nervous system, as demonstrated by in vivo animal studies and by its involvement in the pathogenesis of several human neurocristopathies like Hirschsprung disease and Multiple Endocrine Neoplasia type 2. Using a multistep RT-PCR approach we have isolated and sequenced the cDNA of the whole rat RET proto-oncogene, reporting the deduced amino acid sequence in comparison with the human and mouse counterparts. Moreover, two different isoforms (RET9 and RET51) have been confirmed in the rat, while a third RET isoform demonstrated in human (RET43) has not resulted to be conserved in this species. Finally, we have determined the genomic structure of the rat RET proto-oncogene comparing the exon-intron boundaries and intron sizes with the known structure of the human homologous gene. Our findings will facilitate the molecular study of appropriate rat models of RET related human diseases. |
Agencias financiadoras | Telethon |
Identificador del proyecto | E.0791 |
Cita | Matera, I., Miguel Rodríguez, M.d., Fernández-Santos, J.M., Santamaria, G., Puliti, A., Ravazzolo, R.,...,Ceccherini, I. (2000). cDNA Sequence and Genomic Structure of the Rat Ret Proto-Oncogene. DNA Sequence, 11 (5), 405-417. https://doi.org/10.3109/10425170009033991. |
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