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dc.creatorPérez Aranda, Maríaes
dc.creatorPajuelo Domínguez, Eloísaes
dc.creatorNavarro de la Torre, Salvadoraes
dc.creatorPérez Palacios, Patriciaes
dc.creatorBegines Ruiz, Belénes
dc.creatorRodríguez Llorente, Ignacio Davides
dc.creatorTorres Hernández, Yadires
dc.creatorAlcudia Cruz, Anaes
dc.date.accessioned2023-01-09T16:19:39Z
dc.date.available2023-01-09T16:19:39Z
dc.date.issued2022
dc.identifier.citationPérez Aranda, M., Pajuelo Domínguez, E., Navarro de la Torre, S., Pérez Palacios, P., Begines Ruiz, B., Rodríguez Llorente, I.D.,...,Alcudia Cruz, A. (2022). Antimicrobial and Antibiofilm Effect of 4,4′-Dihydroxy-azobenzene against Clinically Resistant Staphylococci. Antibiotics, 11 (12), 1800. https://doi.org/10.3390/antibiotics11121800.
dc.identifier.issn2079-6382es
dc.identifier.urihttps://hdl.handle.net/11441/141050
dc.description.abstractThe spread of antibiotic resistance among human and animal pathogens is one of the more significant public health concerns. Moreover, the restrictions on the use of particular antibiotics can limit the options for the treatment of infections in veterinary clinical practice. In this context, searching for alternative antimicrobial substances is crucial nowadays. In this study, 4,4′-dihydroxy-azobenzene (DHAB) was tested for its potential in vitro as an antimicrobial agent against two relevant human and animal pathogens, namely Staphylococcus aureus and Staphylococcus pseudintermedius. The values of minimal inhibitory concentration (MIC) were 64 and 32 mg/L respectively, and they comparable to other azo compounds of probed antimicrobial activity. In addition, the minimal bactericidal concentrations (MCB) were 256 and 64 mg/L. The mechanism by which DHAB produces toxicity in staphylococci has been investigated. DHAB caused membrane damage as revealed by the increase in thiobarbituric acid reactive substances (TBARS) such as malondialdehyde. Furthermore, differential induction of the enzymes peroxidases and superoxide dismutase in S. aureus and S. pseudintermedius suggested their prevalent role in ROS-scavenging due to the oxidative burst induced by this compound in either species. In addition, this substance was able to inhibit the formation of biofilms by both bacteria as observed by colorimetric tests and scanning electron microscopy. In order to assess the relevance of DHAB against clinical strains of MRSA, 10 clinical isolates resistant to either methicillin or daptomycin were assayed; 80% of them gave values of CMI and CMB similar to those of the control S. aureus strain. Finally, cutaneous plasters containing a composite formed by an agar base supplemented with DHAB were designed. These plasters were able to inhibit in vitro the growth of S. aureus and S. pseudintermedius, particularly the later, and this suggests that this substance could be a promising candidate as an alternative to antibiotics in the treatment of animal skin infections, as it has been proven that the toxicity of this substance is very low particularly at a dermal level.es
dc.description.sponsorshipMinisterio de Ciencia e Innovación PID2019-109371GB-I00es
dc.description.sponsorshipJunta de Andalucía US-1380878es
dc.formatapplication/pdfes
dc.format.extent18 p.es
dc.language.isoenges
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)es
dc.relation.ispartofAntibiotics, 11 (12), 1800.
dc.rightsAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAzo compoundses
dc.subjectBiofilmses
dc.subjectClinical sampleses
dc.subjectCutaneous plasterses
dc.subjectOxidative stresses
dc.subjectStaphylococcus aureuses
dc.subjectStaphylococcus pseudintermediuses
dc.titleAntimicrobial and Antibiofilm Effect of 4,4′-Dihydroxy-azobenzene against Clinically Resistant Staphylococcies
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiología y Parasitologíaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Química Orgánica y Farmacéuticaes
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transportees
dc.relation.projectIDPID2019-109371GB-I00es
dc.relation.projectIDUS-1380878es
dc.relation.publisherversionhttps://doi.org/10.3390/antibiotics11121800es
dc.identifier.doi10.3390/antibiotics11121800es
dc.journaltitleAntibioticses
dc.publication.volumen11es
dc.publication.issue12es
dc.publication.initialPage1800es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). Españaes
dc.contributor.funderJunta de Andalucíaes

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