The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis
|Author/s||Ayala García, Paula
Jiménez Guerrero, Irene
Jacott, Catherine N.
López Baena, Francisco Javier
Ollero Márquez, Francisco Javier
Cerro Sánchez, Pablo del
Pérez Montaño, Francisco de Asís
|Department||Universidad de Sevilla. Departamento de Microbiología|
|Abstract||In the symbiotic associations between rhizobia and legumes, the NodD regulators orchestrate the transcription of the
specifc nodulation genes. This set of genes is involved in the synthesis of nodulation factors, which ...
In the symbiotic associations between rhizobia and legumes, the NodD regulators orchestrate the transcription of the specifc nodulation genes. This set of genes is involved in the synthesis of nodulation factors, which are responsible for initiating the nodulation process. Rhizobium tropici CIAT 899 is the most successful symbiont of Phaseolus vulgaris and can nodulate a variety of legumes. Among the fve NodD regulators present in this rhizobium, only NodD1 and NodD2 seem to have a role in the symbiotic process. However, the individual role of each NodD in the absence of the other proteins has remained elusive. In this work, we show that the CIAT 899 NodD2 does not require activation by inducers to promote the synthesis of nodulation factors. A CIAT 899 strain overexpressing nodD2, but lacking all additional nodD genes, can nodulate three different legumes as effciently as the wild type. Interestingly, CIAT 899 NodD2- mediated gain of nodulation can be extended to another rhizobial species, since its overproduction in Sinorhizobium fredii HH103 not only increases the number of nitrogen-fxing nodules in two host legumes but also results in nodule development in incompatible legumes. These fndings potentially open exciting opportunities to develop rhizobial inoculants and increase legume crop production.
|Funding agencies||Ministerio de Ciencia e Innovación (MICIN). España
Ministerio de Economía y Competitividad (MINECO). España
|Citation||Ayala García, P., Jiménez Guerrero, I., Jacott, C.N., López Baena, F.J., Ollero Márquez, F.J., Cerro Sánchez, P.d. y Pérez Montaño, F.d.A. (2022). The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis. Journal of Experimental Botany, 73 (19), 6931-6941. https://doi.org/10.1093/jxb/erac325.|