dc.creator | Martín Lozano, Guiomar | es |
dc.creator | Gómez Díaz, Raquel | es |
dc.creator | Iglesias Martín, Fernando | es |
dc.creator | Torres-Lagares, Daniel | es |
dc.creator | Gutiérrez Corrales, Aída | es |
dc.creator | Gutiérrez Pérez, José Luis | es |
dc.date.accessioned | 2022-11-02T17:06:20Z | |
dc.date.available | 2022-11-02T17:06:20Z | |
dc.date.issued | 2021-10 | |
dc.identifier.citation | Martín Lozano, G., Gómez Díaz, R., Iglesias Martín, F., Torres-Lagares, D., Gutiérrez Corrales, A. y Gutiérrez Pérez, J.L. (2021). Mutations in p53 gene exons in a sample from the south of Spain in oral cancer. Journal of Clinical and Experimental Dentistry, 13 (10), e1001-e1005. https://doi.org/10.4317/jced.58799. | |
dc.identifier.issn | 1989-5488 | es |
dc.identifier.uri | https://hdl.handle.net/11441/138618 | |
dc.description.abstract | Background: Cancer is a genetic disease caused by mutations in DNA and epigenetic alterations that control gene
expression. The majority of epidermoid carcinomas develop within the fields of epithelial genetic alterations. The
mechanisms underlying tumorigenesis of epidermoid carcinoma are as yet unknown; therefore, precise identifica tion of the risk factors is needed. Aim: The main aim of this study is to analyse and identify the emergence of the
mutations described in the literature of the p53 gene with regard to the emergence of cancer in a sample of dysplas tic and cancerous lesions in oral cavity mucosa in the population of the south of Spain, in order to determine the
presence of said mutations and the percentage of them in our population.
Material and Methods: A cross-sectional study was carried out, with a sample size of 22 patients with potentially
malignant oral lesions ancillary to biopsy. All were patients, of both sexes, over 18 years of age from the Virgen
del Rocío University Hospital with potentially malignant lesions in oral mucosa ancillary to biopsy (leukoplakias,
erythroplasias or leukoerythopkias). An anatomopathological study was performed on all the samples and the le sions were divided into three types: low-grade dysplasia, high-grade dysplasia and squamous cell carcinoma. In
respect of the genome study process, a complete search or scan for mutations in exons 5, 6, 8 and 9 of the p 53 gene
was carried out, given that in the IARC database we observed that the 5 and 6 as well as the 8 and 9 exon sizes can
be scanned completely in this way, since they have amplificon sizes of 476 and 445 base pairs respectively.
Results: In the scan for the complete exons 5, 6, 8 and 9 only a single result of interest was found to be described. In
patient NBI 57 a change was observed in the TAT triplet by ATT of EXON 6, the change being of the T nucleotide
by the A and in both directions both in Forward and Reverse. The exact location in the NCBI is GR Ch 37 p13 on
chromosome 17, EXON 6 of the P53 gene and the change is in the C.613 T>A nucleotide; NM_000546.
Conclusions: On reviewing this genetic variant in different scientific databases, such as ENSEMBL among others, in
at least 6 different biocomputing tools it is described as a pathogen, therefore we can conclude that it is a pathogenic
mutation for this case in particular. The rest of the mutations described in the literature on exons 5, 6, 8 and 9 of the
p53 gene have not been found in our sample. | es |
dc.format | application/pdf | es |
dc.format.extent | 5 p. | es |
dc.language.iso | eng | es |
dc.publisher | Jose V. Bagan | es |
dc.relation.ispartof | Journal of Clinical and Experimental Dentistry, 13 (10), e1001-e1005. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Oral cancer | es |
dc.subject | p53 | es |
dc.subject | Mutations | es |
dc.subject | Exon | es |
dc.title | Mutations in p53 gene exons in a sample from the south of Spain in oral cancer | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Estomatología | es |
dc.relation.publisherversion | https://doi.org/10.4317/jced.58799 | es |
dc.identifier.doi | 10.4317/jced.58799 | es |
dc.journaltitle | Journal of Clinical and Experimental Dentistry | es |
dc.publication.volumen | 13 | es |
dc.publication.issue | 10 | es |
dc.publication.initialPage | e1001 | es |
dc.publication.endPage | e1005 | es |
dc.contributor.funder | Instituto de Salud Carlos III PI15-01834 | es |