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dc.creatorFeria Bourrellier, Ana Belénes
dc.creatorRuiz Ballesta, Isabeles
dc.creatorBaena Vaca, Guillermoes
dc.creatorRuiz López, Noemies
dc.creatorEchevarría Ruiz de Vargas, Cristinaes
dc.creatorVidal, Jeanes
dc.date.accessioned2022-09-23T17:29:12Z
dc.date.available2022-09-23T17:29:12Z
dc.date.issued2022
dc.identifier.citationFeria Bourrellier, A.B., Ruiz Ballesta, I., Baena Vaca, G., Ruiz López, N., Echevarría Ruiz de Vargas, C. y Vidal, J. (2022). Phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxylase kinase isoenzymes play an important role in the filling and quality of Arabidopsis thaliana seed. Plant Physiology and Biochemistry, 190, 70-80.
dc.identifier.issn0981-9428es
dc.identifier.issn1873-2690es
dc.identifier.urihttps://hdl.handle.net/11441/137356
dc.description.abstractThree plant-type phosphoenolpyruvate carboxylase (PPC1 to PPC3) and two phosphoenolpyruvate carboxylase kinase (PPCKs: PPCK1 and 2) genes are present in the Arabidopsis thaliana genome. In seeds, all PPC genes were found to be expressed. Examination of individual ppc mutants showed little reduction of PEPC protein and global activity, with the notable exception of PPC2 which represent the most abundant PEPC in dry seeds. Ppc mutants exhibited moderately lower seed parameters (weight, area, yield, germination kinetics) than wild type. In contrast, ppck1-had much altered (decreased) yield. At the molecular level, ppc3-was found to be significantly deficient in global seed nitrogen (nitrate, amino-acids, and soluble protein pools). Also, N-deficiency was much more marked in ppck1-, which exhibited a tremendous loss of 95% and 90% in nitrate and proteins, respectively. The line ppck2-had accumulated amino-acids but lower levels of soluble proteins. Regarding carboxylic acid pools, Krebs cycle intermediates were found to be diminished in all mutants; this was accompanied by a consistent decrease in ATP. Lipids were stable in ppc mutants, however ppck1-seeds accumulated more lipids while ppck2-seeds showed high level of polyunsaturated fatty acid oleic and linolenic (omega 3). Altogether, the results indicate that the complete PEPC and PPCK family are needed for normal C/N metabolism ratio, growth, development, yield and quality of the seed.es
dc.description.sponsorshipMinisterio de Economía y Competitividad AGL2012-35708, AGL2016-75413-Pes
dc.description.sponsorshipJunta de Andalucía P12-FQM-489es
dc.formatapplication/pdfes
dc.format.extent11 p.es
dc.language.isoenges
dc.publisherElsevieres
dc.relation.ispartofPlant Physiology and Biochemistry, 190, 70-80.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectAnaplerotic functiones
dc.subjectGerminationes
dc.subjectPhosphoenolpyruvate carboxylasees
dc.subjectPhosphoenolpyruvate carboxylase kinasees
dc.subjectSeed yield and qualityes
dc.subjectT-DNA mutantses
dc.titlePhosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxylase kinase isoenzymes play an important role in the filling and quality of Arabidopsis thaliana seedes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Biología Vegetal y Ecologíaes
dc.relation.projectIDAGL2012-35708es
dc.relation.projectIDAGL2016-75413-Pes
dc.relation.projectIDP12-FQM-489es
dc.relation.publisherversionhttps://doi.org/10.1016/j.plaphy.2022.08.012es
dc.identifier.doi10.1016/j.plaphy.2022.08.012es
dc.journaltitlePlant Physiology and Biochemistryes
dc.publication.volumen190es
dc.publication.initialPage70es
dc.publication.endPage80es
dc.contributor.funderMinisterio de Economía y Competitividad (MINECO). Españaes
dc.contributor.funderJunta de Andalucíaes

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