dc.creator | Huang, Yue | es |
dc.creator | Liu, Yuan | es |
dc.creator | Shah, Shrey | es |
dc.creator | Kim, Dongyeop | es |
dc.creator | Simón Soro, Áurea | es |
dc.creator | Ito, Tatsuro | es |
dc.creator | Koo, Hyun | es |
dc.date.accessioned | 2022-07-21T10:45:00Z | |
dc.date.available | 2022-07-21T10:45:00Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Huang, Y., Liu, Y., Shah, S., Kim, D., Simón-Soro, Á., Ito, T. y Koo, H. (2021). Precision targeting of bacterial pathogen via bi-functional nanozyme activated by biofilm microenvironment. Biomaterials, 268 (120581) | |
dc.identifier.issn | 0142-9612 | es |
dc.identifier.issn | 1878-5905 | es |
dc.identifier.uri | https://hdl.handle.net/11441/135688 | |
dc.description.abstract | Human dental caries is an intractable biofilm-associated disease caused by microbial interactions and dietary sugars on the host's teeth. Commensal bacteria help control opportunistic pathogens via bioactive products such as hydrogen peroxide (H2O2). However, high-sugar consumption disrupts homeostasis and promotes pathogen accumulation in acidic biofilms that cause tooth-decay. Here, we exploit the pathological (sugar-rich/acidic) conditions using a nanohybrid system to increase intrinsic H2O2 production and trigger pH-dependent reactive oxygen species (ROS) generation for efficient biofilm virulence targeting. The nanohybrid contains glucose-oxidase that catalyzes glucose present in biofilms to increase intrinsic H2O2, which is converted by iron oxide nanoparticles with peroxidase-like activity into ROS in acidic pH. Notably, it selectively kills Streptococcus mutans (pathogen) without affecting Streptococcus oralis (commensal) via preferential pathogen-binding and in situ ROS generation. Furthermore, nanohybrid treatments potently reduced dental caries in a rodent model. Compared to chlorhexidine (positive-control), which disrupted oral microbiota diversity, the nanohybrid had significant higher efficacy without affecting soft-tissues and the oral-gastrointestinal microbiomes, while modulating dental health-associated microbial activity in vivo. The data reveal therapeutic precision of a bi-functional hybrid nanozyme against a biofilm-related disease in a controlled-manner activated by pathological conditions. | es |
dc.description.sponsorship | National Institutes of Health/ National Institute of Dental and Craniofacial Research | es |
dc.format | application/pdf | es |
dc.format.extent | 12 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier LTD. | es |
dc.relation.ispartof | Biomaterials, 268 (120581) | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Hybrid nanozyme | es |
dc.subject | Glucose oxidase | es |
dc.subject | Catalytic nanoparticles | es |
dc.subject | Biofilm | es |
dc.subject | Polymicrobial | es |
dc.subject | Dental caries | es |
dc.title | Precision targeting of bacterial pathogen via bi-functional nanozyme activated by biofilm microenvironment | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Estomatología | es |
dc.relation.projectID | R01DE025848 | es |
dc.relation.publisherversion | https://reader.elsevier.com/reader/sd/pii/S0142961220308279?token=80DB3AA16006D777FF8E3DC6A33D55AF84956F1E0234398A19A34BF99588C19A89CFD21862FD92C2867162EAF75BCE56&originRegion=eu-west-1&originCreation=20220721103113 | es |
dc.identifier.doi | 10.1016/j.biomaterials.2020.120581 | es |
dc.journaltitle | Biomaterials | es |
dc.publication.volumen | 268 | es |
dc.publication.issue | 120581 | es |