dc.creator | Ugarte Uribe, Begoña | es |
dc.creator | Pérez Rentero, Sonia | es |
dc.creator | Lucas Rodríguez, Ricardo | es |
dc.creator | Aviñó, Anna | es |
dc.creator | Reina, José Juan | es |
dc.creator | Alkorta, Itziar | es |
dc.creator | Eritja, Ramón | es |
dc.creator | Morales, Juan Carlos | es |
dc.date.accessioned | 2022-07-13T14:11:18Z | |
dc.date.available | 2022-07-13T14:11:18Z | |
dc.date.issued | 2010 | |
dc.identifier.citation | Ugarte Uribe, B., Pérez Rentero, S., Lucas Rodríguez, R., Aviñó, A., Reina, J.J., Alkorta, I.,...,Morales, J.C. (2010). Synthesis, cell-surface binding, and cellular uptake of fluorescently labeled glucose-DNA conjugates with different carbohydrate presentation. Bioconjugate Chemistry, 21 (7), 1280-1287. | |
dc.identifier.issn | 1043-1802 | es |
dc.identifier.issn | 1520-4812 | es |
dc.identifier.uri | https://hdl.handle.net/11441/135319 | |
dc.description.abstract | Oligonucleotide conjugates carrying carbohydrates at the 5′-end have been prepared. Glucose, fucose, and saccharides containing glucose at the nonreducing end were attached to DNA strands using the classical phosphoramidite chemistry. Two types of spacers and a dendron scaffold helped to obtain a diversity of sugar presentations in the DNA conjugates. Cellular surface adsorption and cellular uptake of carbohydrate oligonucleotide antisense sequences were measured using flow cytometric analysis. Conjugates with the glucose moiety linked through long spacers (15 to 18 atom distances) were internalized better than those with short linkers (4 atom distance) and than DNA control strands without sugar modification. Conjugates with tetravalent presentation of glucose did not improve cell uptake. | es |
dc.description.sponsorship | Consejo Superior de Investigaciones Científicas PIF06-045 | es |
dc.description.sponsorship | Ministerio de Educación BFU2007-63287, BFU2007-62062, CTQ2006-01123/BQU | es |
dc.description.sponsorship | Generalitat de Catalunya 2009/SGR/208 | es |
dc.description.sponsorship | Instituto de Salud Carlos III CB06_01_0019 | es |
dc.format | application/pdf | es |
dc.format.extent | 27 p. | es |
dc.language.iso | eng | es |
dc.publisher | American Chemical Society | es |
dc.relation.ispartof | Bioconjugate Chemistry, 21 (7), 1280-1287. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Synthesis, cell-surface binding, and cellular uptake of fluorescently labeled glucose-DNA conjugates with different carbohydrate presentation | es |
dc.type | info:eu-repo/semantics/article | es |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica | es |
dc.relation.projectID | PIF06-045 | es |
dc.relation.projectID | BFU2007-63287 | es |
dc.relation.projectID | BFU2007-62062 | es |
dc.relation.projectID | CTQ2006-01123/BQU | es |
dc.relation.projectID | 2009/SGR/208 | es |
dc.relation.projectID | CB06_01_0019 | es |
dc.relation.publisherversion | https://doi.org/10.1021/bc100079n | es |
dc.identifier.doi | 10.1021/bc100079n | es |
dc.journaltitle | Bioconjugate Chemistry | es |
dc.publication.volumen | 21 | es |
dc.publication.issue | 7 | es |
dc.publication.initialPage | 1280 | es |
dc.publication.endPage | 1287 | es |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (CSIC) | es |
dc.contributor.funder | Ministerio de Educación. España | es |
dc.contributor.funder | Generalitat de Catalunya | es |
dc.contributor.funder | Instituto de Salud Carlos III | es |