dc.creator | Aviñó, Anna | es |
dc.creator | Ocampo, Sandra M. | es |
dc.creator | Lucas Rodríguez, Ricardo | es |
dc.creator | Reina, José Juan | es |
dc.creator | Morales, Juan Carlos | es |
dc.creator | Perales, José Carlos | es |
dc.creator | Eritja, Ramón | es |
dc.date.accessioned | 2022-07-13T13:41:45Z | |
dc.date.available | 2022-07-13T13:41:45Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Aviñó, A., Ocampo, S.M., Lucas Rodríguez, R., Reina, J.J., Morales, J.C., Perales, J.C. y Eritja, R. (2011). Synthesis and in vitro inhibition properties of siRNA conjugates carrying glucose and galactose with different presentations. Molecular Diversity, 15 (3), 751-757. | |
dc.identifier.issn | 1381-1991 | es |
dc.identifier.issn | 1573-501X | es |
dc.identifier.uri | https://hdl.handle.net/11441/135318 | |
dc.description.abstract | Oligoribonucleotide conjugates and the corresponding siRNA duplexes against tumor necrosis factor carrying one, two, or four glucose and galactose residues at the 5'-end have been prepared using phosphoramidite chemistry. Carbohydrate-modified siRNA duplexes have similar inhibitory properties than unmodified RNA duplexes in HeLa cells transfected with oligofectamine. When HeLa cells were treated with siRNA carrying one, two, or four glucose residues without oligofectamine, no inhibition was observed. The inhibitory properties of siRNA carrying galactose residues without transfecting agent were tested on HuH-7 cells that have abundant asialoglycoprotein receptors. In these cells siRNA carrying galactose residues have slight anti-TNF inhibitory properties (25% in the best case) that are eliminated if the receptors are blocked with a competitor. These results demonstrate receptor-mediated uptake of siRNA carrying galactose residues, although the efficacy of the process is not enough for efficient RNA interference experiments. | es |
dc.description.sponsorship | Consejo Superior de Investigaciones Científicas PIF06- 045 | es |
dc.description.sponsorship | Ministerio de Educación BFU2007-63287, CTQ2006-01123, CTQ2010-20541, CTQ2009-13705 | es |
dc.description.sponsorship | Generalitat de Catalunya 2009/SGR/208 | es |
dc.format | application/pdf | es |
dc.format.extent | 26 p. | es |
dc.language.iso | eng | es |
dc.publisher | Springer Nature | es |
dc.relation.ispartof | Molecular Diversity, 15 (3), 751-757. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Oligonucleotide synthesis | es |
dc.subject | Oligonucleotide-carbohydrate conjugates | es |
dc.subject | RNA interference | es |
dc.subject | siRNA | es |
dc.subject | Tumor necrosis factor | es |
dc.title | Synthesis and in vitro inhibition properties of siRNA conjugates carrying glucose and galactose with different presentations | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica | es |
dc.relation.projectID | PIF06- 045 | es |
dc.relation.projectID | BFU2007-63287 | es |
dc.relation.projectID | CTQ2006-01123 | es |
dc.relation.projectID | CTQ2010-20541 | es |
dc.relation.projectID | CTQ2009-13705 | es |
dc.relation.projectID | 2009/SGR/208 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1007/s11030-011-9305-6 | es |
dc.identifier.doi | 10.1007/s11030-011-9305-6 | es |
dc.journaltitle | Molecular Diversity | es |
dc.publication.volumen | 15 | es |
dc.publication.issue | 3 | es |
dc.publication.initialPage | 751 | es |
dc.publication.endPage | 757 | es |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (CSIC) | es |
dc.contributor.funder | Ministerio de Educación. España | es |
dc.contributor.funder | Generalitat de Catalunya | es |