dc.creator | Mama, Olouwafemi Mistourath | es |
dc.creator | Aspiroz, Carmen | es |
dc.creator | Ruiz-Ripa, Laura | es |
dc.creator | Ceballos, Sara | es |
dc.creator | Iñiguez Barrio, Maria | es |
dc.creator | Cercenado, Emilia | es |
dc.creator | Azcona, José Manuel | es |
dc.creator | López Cerero, Lorena | es |
dc.creator | Seral, Cristina | es |
dc.creator | Torres, Carmen | es |
dc.date.accessioned | 2022-07-13T11:41:53Z | |
dc.date.available | 2022-07-13T11:41:53Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Mama, O.M., Aspiroz, C., Ruiz-Ripa, L., Ceballos, S., Iñiguez Barrio, M., Cercenado, E.,...,Torres, C. (2021). Prevalence and Genetic Characteristics of Staphylococcus aureus CC398 Isolates From Invasive Infections in Spanish Hospitals, Focusing on the Livestock-Independent CC398-MSSA Clade. Frontiers in Microbiology, 12, 623108. | |
dc.identifier.issn | 1664-302X | es |
dc.identifier.uri | https://hdl.handle.net/11441/135316 | |
dc.description.abstract | Background: Livestock-associated (LA)-CC398-MRSA is closely related to pigs, being unfrequently detected in human invasive infections. CC398-MSSA is emerging in human invasive infections in some countries, but genetic and epidemiological characteristics are still scarcely reported.
Objectives: To determine the prevalence of Staphylococcus aureus (SA) CC398, both MRSA and MSSA, among blood cultures SA isolates recovered in Spanish hospitals located in regions with different pig-farming densities (PD) and characterize the recovered isolates.
Methods: One thousand twenty-two SA isolates (761 MSSA, 261 MRSA) recovered from blood cultures during 6–12 months in 17 Spanish hospitals (2018–2019) were studied. CC398 lineage identification, detection of spa-types, and antibiotic resistance, virulence and human immune evasion cluster (IEC) genes were analyzed by PCR/sequencing.
Results: Forty-four CC398-MSSA isolates (4.3% of SA; 5.8% of MSSA) and 10 CC398-MRSA isolates (1% of SA; 3.8% of MRSA) were detected. Eleven spa-types were found among the CC398-MSSA isolates with t571 and t1451 the most frequent spa-types detected (75%). Most of CC398-MSSA isolates were Immune-Evasion-Cluster (IEC)-positive (88.6%), tetracycline-susceptible (95.5%) and erythromycin/clindamycin–inducible-resistant/erm(T)-positive (75%). No statistical significance was detected when the CC398-MSSA/MSSA rate was correlated to PD (pigs/km2) (p = 0.108). On the contrary, CC398-MRSA isolates were all IEC-negative, predominately spa-t011 (70%), and the CC398-MRSA/MRSA rate was significantly associated to PD (p < 0.005).
Conclusion: CC398-MSSA is an emerging clade in invasive infections in Spanish hospitals. CC398-MRSA (mostly t011) and CC398-MSSA (mostly t571 and t1451) show important differences, possibly suggesting divergent steps in host-adaptation evolutionary processes. While CC398-MRSA is livestock-associated (lacking IEC-system), CC398-MSSA seems to be mostly livestock-independent, carrying human-adaptation markers. | es |
dc.description.sponsorship | Agencia Estatal de Investigación (AEI) (project SAF2016-76571- R) and by the Fondo Europeo de Desarrollo Regional (FEDER) | es |
dc.format | application/pdf | es |
dc.format.extent | 10 p. | es |
dc.language.iso | eng | es |
dc.publisher | Frontiers Media | es |
dc.relation.ispartof | Frontiers in Microbiology, 12, 623108. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | MSSA | es |
dc.subject | LA-MRSA | es |
dc.subject | CC398 | es |
dc.subject | Bacteremia | es |
dc.subject | t571 | es |
dc.subject | t1451 | es |
dc.subject | erm(T) | es |
dc.subject | Spain | es |
dc.title | Prevalence and Genetic Characteristics of Staphylococcus aureus CC398 Isolates From Invasive Infections in Spanish Hospitals, Focusing on the Livestock-Independent CC398-MSSA Clade | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Microbiología | es |
dc.relation.projectID | SAF2016-76571-R | es |
dc.relation.publisherversion | https://dx.doi.org/10.3389/fmicb.2021.623108 | es |
dc.identifier.doi | 10.3389/fmicb.2021.623108 | es |
dc.journaltitle | Frontiers in Microbiology | es |
dc.publication.volumen | 12 | es |
dc.publication.initialPage | 623108 | es |
dc.contributor.funder | Agencia Estatal de Investigación. España | es |
dc.contributor.funder | European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) | es |