dc.creator | Vengut Climent, Empar | es |
dc.creator | Terrazas, Montserrat | es |
dc.creator | Lucas Rodríguez, Ricardo | es |
dc.creator | Arévalo Ruiz, Matilde | es |
dc.creator | Eritja, Ramón | es |
dc.creator | Morales, Juan Carlos | es |
dc.date.accessioned | 2022-07-12T15:04:22Z | |
dc.date.available | 2022-07-12T15:04:22Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Vengut Climent, E., Terrazas, M., Lucas Rodríguez, R., Arévalo Ruiz, M., Eritja, R. y Morales, J.C. (2013). Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates. Bioorganic and Medicinal Chemistry Letters, 23 (14), 4048-4051. | |
dc.identifier.issn | 0960-894X | es |
dc.identifier.issn | 1464-3405 | es |
dc.identifier.uri | https://hdl.handle.net/11441/135277 | |
dc.description.abstract | Oligoribonucleotide conjugates carrying apolar carbohydrates at the 5′-end and the corresponding siRNA duplexes have been prepared using phosphoramidite chemistry. All the carbohydrate-siRNA derivatives were compatible with RNA interference machinery if transfected with oligofectamine. In the absence of a transfection agent, some of them exerted certain reduction of gene expression. Double-tailed permethylated glucose conjugated to siRNA through a long spacer inhibited gene expression up to 26% compared to the scrambled duplex. Such modifications contribute positively to the stability of oligoribonucleotides against 5′-exonuclease degradation. | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad CTQ2009-13705, CTQ2010-20541 | es |
dc.description.sponsorship | Generalitat de Catalunya 2009/SGR/208 | es |
dc.format | application/pdf | es |
dc.format.extent | 8 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Bioorganic and Medicinal Chemistry Letters, 23 (14), 4048-4051. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Carbohydrate | es |
dc.subject | Lipophilic vector | es |
dc.subject | Modified siRNA | es |
dc.subject | Phosphoramidite | es |
dc.subject | siRNA | es |
dc.title | Synthesis, RNAi activity and nuclease-resistant properties of apolar carbohydrates siRNA conjugates | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica | es |
dc.relation.projectID | CTQ2009-13705 | es |
dc.relation.projectID | CTQ2010-20541 | es |
dc.relation.projectID | 2009/SGR/208 | es |
dc.relation.publisherversion | https://doi.org/10.1016/j.bmcl.2013.05.065 | es |
dc.identifier.doi | 10.1016/j.bmcl.2013.05.065 | es |
dc.journaltitle | Bioorganic and Medicinal Chemistry Letters | es |
dc.publication.volumen | 23 | es |
dc.publication.issue | 14 | es |
dc.publication.initialPage | 4048 | es |
dc.publication.endPage | 4051 | es |
dc.contributor.funder | Ministerio de Economía y Competitividad (MINECO). España | es |
dc.contributor.funder | Generalitat de Catalunya | es |