dc.creator | Gallardo Morillo, Elena | es |
dc.creator | Sarriá, Beatriz | es |
dc.creator | Espartero Sánchez, José Luis | es |
dc.creator | Gonzalez Correa, José Antonio | es |
dc.creator | Bravo Clemente, Laura | es |
dc.creator | Mateos, Raquel | es |
dc.date.accessioned | 2022-07-11T16:20:24Z | |
dc.date.available | 2022-07-11T16:20:24Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Gallardo Morillo, E., Sarriá, B., Espartero Sánchez, J.L., Gonzalez Correa, J.A., Bravo Clemente, L. y Mateos, R. (2016). Evaluation of the Bioavailability and Metabolism of Nitroderivatives of Hydroxytyrosol Using Caco-2 and HepG2 Human Cell Models. Journal of Agricultural and Food Chemistry, 64 (11), 2289-2297. | |
dc.identifier.issn | 0021-8561 | es |
dc.identifier.issn | 1520-5118 | es |
dc.identifier.uri | https://hdl.handle.net/11441/135228 | |
dc.description.abstract | Considering that nitrocatechols present putative effects against Parkinson's disease, the absorption and metabolism of nitroderivatives of hydroxytyrosol (HT) were assessed using human cell model systems. The test compounds nitrohydroxytyrosol (NO2HT), nitrohydroxytyrosyl acetate (NO2HT-A), and ethyl nitrohydroxytyrosyl ether (NO2HT-E) were efficiently transferred across human Caco-2 cell monolayers as an intestinal barrier model, NO2HT-A and NO2HT-E being better (p < 0.05) absorbed (absorption rate (AR) = 1.4 ± 0.1 and 1.5 ± 0.2, respectively) than their precursor, NO2HT (AR = 1.1 ± 0.1). A significant amount of the absorbed compounds remained unconjugated (81, 70, and 33% for NO2HT, NO2HT-A, and NO2HT-E, respectively) after incubation in Caco-2 cells, being available for hepatic metabolism. Nitrocatechols were extensively taken up and metabolized by human hepatoma HepG2 cells as a model of the human liver. Both studies revealed extensive hydrolysis of NO2HT-A into NO2HT, whereas NO2HT-E was not hydrolyzed. Glucuronide (75-55%), methylglucuronide (25-33%), and methyl derivatives (0-12%) were the main nitrocatechol metabolites detected after metabolism in Caco-2 and HepG2 cells. In conclusion, NO2HT, NO2HT-A, and NO2HT-E show high in vitro bioavailability and are extensively metabolized by hepatic cells. | es |
dc.description.sponsorship | Junta de Andalucía P09-AGR-5098 | es |
dc.format | application/pdf | es |
dc.format.extent | 32 p. | es |
dc.language.iso | eng | es |
dc.publisher | American Chemical Society | es |
dc.relation.ispartof | Journal of Agricultural and Food Chemistry, 64 (11), 2289-2297. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Bioavailability | es |
dc.subject | Hydroxytyrosol | es |
dc.subject | Metabolism | es |
dc.subject | Nitrocatechols | es |
dc.subject | Parkinson's disease | es |
dc.title | Evaluation of the Bioavailability and Metabolism of Nitroderivatives of Hydroxytyrosol Using Caco-2 and HepG2 Human Cell Models | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica | es |
dc.relation.projectID | P09-AGR-5098 | es |
dc.relation.publisherversion | https://doi.org/10.1021/acs.jafc.6b00401 | es |
dc.identifier.doi | 10.1021/acs.jafc.6b00401 | es |
dc.journaltitle | Journal of Agricultural and Food Chemistry | es |
dc.publication.volumen | 64 | es |
dc.publication.issue | 11 | es |
dc.publication.initialPage | 2289 | es |
dc.publication.endPage | 2297 | es |
dc.contributor.funder | Junta de Andalucía | es |