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dc.creatorRivero Fernández, Juliaes
dc.creatorZurita Carrasco, Antonioes
dc.creatorCutillas Barrios, Cristinaes
dc.creatorCallejón Fernández, Rocíoes
dc.date.accessioned2022-06-10T08:31:03Z
dc.date.available2022-06-10T08:31:03Z
dc.date.issued2022
dc.identifier.citationRivero Fernández, J., Zurita Carrasco, A., Cutillas Barrios, C. y Callejón Fernández, R. (2022). The Use of MALDI-TOF MS as a Diagnostic Tool for Adult Trichuris Species. Frontiers in Veterinary Science, 9 (13 p.), 867919.
dc.identifier.issn2297-1769es
dc.identifier.urihttps://hdl.handle.net/11441/134268
dc.description.abstractTrichuriasis is considered a neglected tropical disease, being the second most common helminthiasis in humans. Detection of Trichuris in routine diagnosis is usually done by microscopic detection of eggs in fecal samples. Other molecular analyses are more reliable and could be used, but these analyses are not routinely available in clinical microbiology laboratories. The use of matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) is increasing since the last decades due to its recent evidence as a potential role for reliable identification of microorganisms and a few nematodes. But, for parasites detection, normalized protocols and the acquisition and introduction of new species to the database are required. We carried out a preliminary study confirming the usefulness of MALDI-TOF MS for the rapid and reliable identification of Trichuris suis used as control and the creation of an internal database. To create main spectra profiles (MSPs), the different parts of five whipworms (esophagus and intestine) were used, developing different tests to verify the repeatability and reproducibility of the spectra. Thus, to validate the new internal database, 20 whipworms, separating the esophagus and intestine, were used, of which 100% were accurately identified as T. suis, but could not distinguish between both parts of the worm. Log score values ranged between 1.84 and 2.36, meaning a high-quality identification. The results confirmed that MALDI-TOF MS was able to identify Trichuris species. Additionally, a MALDI-TOF MS profile of T. suis proteome was carried out to develop the first internal database of spectra for the diagnosis of trichuriasis and other Trichuris spp.es
dc.description.sponsorshipEspaña MCIN/AEI/10.13039/501100011033 Grant (CGL2017- 83057)es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherFrontierses
dc.relation.ispartofFrontiers in Veterinary Science, 9 (13 p.), 867919.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectTrichurises
dc.subjectMALDI-TOF MSes
dc.subjectdiagnosises
dc.subjectnematodees
dc.subjectinternal databasees
dc.titleThe Use of MALDI-TOF MS as a Diagnostic Tool for Adult Trichuris Specieses
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessRightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiología y Parasitologíaes
dc.relation.projectIDCGL2017- 83057es
dc.relation.publisherversionhttps://dx.doi.org/10.3389/fvets.2022.867919es
dc.identifier.doi10.3389/fvets.2022.867919es
dc.journaltitleFrontiers in Veterinary Sciencees
dc.publication.volumen9es
dc.publication.issue13 p.es
dc.publication.endPage867919es
dc.contributor.funderMinisterio de Ciencia e Innovación (MICIN). Españaes

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