Artículo
Pairing statistics and melting of random DNA oligomers: Finding your partner in superdiverse environments
Autor/es | Leo, Simone Di
Marni, Stefano Plata Ramos, Carlos Alberto Fraccia, Tommaso P. Smith, Gregory P. Maritan, Amos Suweis, Samir Bellini, Tommaso |
Departamento | Universidad de Sevilla. Departamento de Física Atómica, Molecular y Nuclear |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-06-08 |
Publicado en |
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Resumen | Understanding of the pairing statistics in solutions populated by a large number of distinct solute species with mutual interactions is a challenging topic, relevant in modeling the complexity of real biological systems. ... Understanding of the pairing statistics in solutions populated by a large number of distinct solute species with mutual interactions is a challenging topic, relevant in modeling the complexity of real biological systems. Here we describe, both experimentally and theoretically, the formation of duplexes in a solution of random-sequence DNA (rsDNA) oligomers of length L = 8, 12, 20 nucleotides. rsDNA solutions are formed by 4L distinct molecular species, leading to a variety of pairing motifs that depend on sequence complementarity and range from strongly bound, fully paired defectless helices to weakly interacting mismatched duplexes. Experiments and theory coherently combine revealing a hybridization statistics characterized by a prevalence of partially defected duplexes, with a distribution of type and number of pairing errors that depends on temperature. We find that despite the enormous multitude of inter-strand interactions, defectless duplexes are formed, involving a fraction up to 15% of the rsDNA chains at the lowest temperatures. Experiments and theory are limited here to equilibrium conditions. |
Cita | Leo, S.D., Marni, S., Plata Ramos, C.A., Fraccia, T.P., Smith, G.P., Maritan, .,...,Bellini, T. (2022). Pairing statistics and melting of random DNA oligomers: Finding your partner in superdiverse environments. Plos Computational Biology, 18 (4), e1010051. |
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pubpcbi.1010051.pdf | 3.074Mb | [PDF] | Ver/ | |