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Artículo
Quantitative analysis of protein-RNA interactions in fission yeast
Autor/es | Elías Villalobos, Alberto
Duncan, Caia Mata, Juan Helmlinger, Dominique |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2022 |
Fecha de depósito | 2022-05-27 |
Publicado en |
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Resumen | Characterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein ... Characterizing the interactions between RNAs and proteins in vivo is key to better understand how organisms regulate gene expression. Here, we describe a robust and quantitative protocol to measure specific RNA-protein interactions in a native context using RNA immunoprecipitation (RIP). We provide a comprehensive experimental framework to detect cotranslational interactions and detail the quantitative analysis of purified RNAs by PCR and high-throughput sequencing. Although we developed the protocol in fission yeast, it can be readily implemented in other yeast species. For complete details on the use and execution of this protocol, please refer to Toullec et al. (2021). |
Agencias financiadoras | Fondation pour la Recherche Médicale Biotechnology and Biological Sciences Research Council (BBSRC) Agence Nationale de la Recherche. France Fondation ARC pour la recherche sur le cancer |
Identificador del proyecto | SPF20130526854
BB/G011869/1 BB/S015833/1 ANR-15-CE12-0009-01 ANR-15-CE11-0022-03 PJA 20181208277 |
Cita | Elías Villalobos, A., Duncan, C., Mata, J. y Helmlinger, D. (2022). Quantitative analysis of protein-RNA interactions in fission yeast. STAR Protocols, 3 (2), 101373. |
Ficheros | Tamaño | Formato | Ver | Descripción |
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Quantitative analysis.pdf | 3.331Mb | [PDF] | Ver/ | |