dc.creator | El Hammadi, Mazen M. | es |
dc.creator | Small Howard, Andrea L. | es |
dc.creator | Jansen, Chad | es |
dc.creator | Fernández Arévalo, María Mercedes | es |
dc.creator | Turner, Helen | es |
dc.creator | Martín Banderas, Lucía | es |
dc.date.accessioned | 2022-03-25T12:36:38Z | |
dc.date.available | 2022-03-25T12:36:38Z | |
dc.date.issued | 2022 | |
dc.identifier.citation | El Hammadi, M.M., Small Howard, A.L., Jansen, C., Fernández Arévalo, M.M., Turner, H. y Martín Banderas, L. (2022). Potential use for chronic pain: Poly(Ethylene Glycol)-Poly(Lactic-Co-Glycolic Acid) nanoparticles enhance the effects of Cannabis-Based terpenes on calcium influx in TRPV1-Expressing cells. International Journal of Pharmaceutics, 616, 121524. | |
dc.identifier.issn | 0378-5173 | es |
dc.identifier.uri | https://hdl.handle.net/11441/131294 | |
dc.description.abstract | The objective of these in vitro studies was to investigate the impact of the encapsulation of three cannabis-based terpenes, namely β-myrcene (MC), β-caryophyllene (CPh), and nerolidol (NL), on their potential efficacy in pain management. Terpene-encapsulated poly(ethylene glycol)-poly(lactic-co-glycolic acid) nanoparticles (PEG-PLGA NPs) were prepared by an emulsion-solvent evaporation method. The terpene-loaded NPs were examined in HEK293 cells that express the nociceptive transient receptor potential vanilloid- 1 (TRPV1), an ion channel involved in pain perception. TRPV1 activation was assessed by monitoring calcium influx kinetics over 1 h in cells pre-treated with the fluorescent indicator Fluo-4. In addition, the fluorescence intensity changes induced by the NPs in living cells were also explored by a fluorescence microscope. Furthermore, the cytotoxicity of the terpene-loaded NPs was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl tetrazolium bromide (MTT) proliferation assay. The terpene-loaded NPs had a diameter in the range of 250–350 nm and a zeta potential of approximately −20 mV. The encapsulation efficiency was 18.5%, 51.3%, and 60.3% for MC, NL, and CPh NPs, respectively. The nano-formulations significantly increased the fluorescence intensity in comparison with free terpenes. Furthermore, combinations of terpene-loaded NPs produced significantly higher calcium responses when compared to combinations of free terpenes. Similar findings were shown by the fluorescence images. In conclusion, the terpene-PLGA NPs can be promising therapeutics for more effective pain management. | es |
dc.format | application/pdf | es |
dc.format.extent | 8 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | International Journal of Pharmaceutics, 616, 121524. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cannabis-based terpenes | es |
dc.subject | PLGA polymeric nanoparticles | es |
dc.subject | Beta-myrcene | es |
dc.subject | Nerolidol | es |
dc.subject | Beta-caryophyllene | es |
dc.subject | Nanomedicine | es |
dc.subject | Chronic pain | es |
dc.title | Potential use for chronic pain: Poly(Ethylene Glycol)-Poly(Lactic-Co-Glycolic Acid) nanoparticles enhance the effects of Cannabis-Based terpenes on calcium influx in TRPV1-Expressing cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.ijpharm.2022.121524 | es |
dc.identifier.doi | 10.1016/j.ijpharm.2022.121524 | es |
dc.journaltitle | International Journal of Pharmaceutics | es |
dc.publication.volumen | 616 | es |
dc.publication.initialPage | 121524 | es |