Artículo
Adaptation of Salmonella enterica to bile: essential role of AcrAB-mediated efflux
Autor/es | Urdaneta Páez, Verónica Carolina
Casadesús Pursals, Josep |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2018 |
Fecha de depósito | 2022-03-21 |
Publicado en |
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Resumen | Adaptation to bile is the ability to endure the lethal effects of bile salts after growth on sublethal concentrations. Surveys of adaptation to bile in Salmonella enterica ser. Tyhimurium reveal that active efflux is ... Adaptation to bile is the ability to endure the lethal effects of bile salts after growth on sublethal concentrations. Surveys of adaptation to bile in Salmonella enterica ser. Tyhimurium reveal that active efflux is essential for adaptation while other bacterial functions involved in bile resistance are not. Among S. enterica mutants lacking one or more efflux systems, only strains lacking AcrAB are unable to adapt, thus revealing an essential role for AcrAB. Transcription of the acrAB operon is upregulated in the presence of a sublethal concentration of sodium deoxycholate (DOC) while other efflux loci are either weakly upregulated or irresponsive. Upregulation of acrAB transcription is strong during exponential growth, and weak in stationary cultures. Single cell analysis of ethidium bromide accumulation indicates that DOC-induced AcrAB-mediated efflux occurs in both exponential and stationary cultures. Upregulation of acrAB expression may thus be crucial at early stages of adaptation, while sustained AcrAB activity may be sufficient to confer bile resistance in nondividing cells. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER) Junta de Andalucía |
Identificador del proyecto | BIO2013–44220-R
BIO2016–75235-P CVI-5879 |
Cita | Urdaneta Páez, V.C. y Casadesús Pursals, J. (2018). Adaptation of Salmonella enterica to bile: essential role of AcrAB-mediated efflux. Environmental Microbiology, 20 (4), 1405-1418. |
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