Artículo
NleB/SseK-catalyzed arginine-glycosylation and enteropathogen virulence are finely tuned by a single variable position contiguous to the catalytic machinery
Autor/es | García García, Ana
Hicks, Thomas El Qaidi, Samir Zhu, Congrui Hardwidge, Philip R. Angulo Álvarez, Jesús Hurtado Guerrero, Ramón |
Departamento | Universidad de Sevilla. Departamento de Química orgánica |
Fecha de publicación | 2021 |
Fecha de depósito | 2022-03-09 |
Publicado en |
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Resumen | NleB/SseK effectors are arginine-GlcNAc-transferases expressed by enteric bacterial pathogens that modify host cell proteins to disrupt signaling pathways. While the conserved Citrobacter rodentium NleB and E. coli NleB1 ... NleB/SseK effectors are arginine-GlcNAc-transferases expressed by enteric bacterial pathogens that modify host cell proteins to disrupt signaling pathways. While the conserved Citrobacter rodentium NleB and E. coli NleB1 proteins display a broad selectivity towards host proteins, Salmonella enterica SseK1, SseK2, and SseK3 have a narrowed protein substrate selectivity. Here, by combining computational and biophysical experiments, we demonstrate that the broad protein substrate selectivity of NleB relies on Tyr284NleB/NleB1, a second-shell residue contiguous to the catalytic machinery. Tyr284NleB/NleB1 is important in coupling protein substrate binding to catalysis. This is exemplified by S286YSseK1 and N302YSseK2 mutants, which become active towards FADD and DR3 death domains, respectively, and whose kinetic properties match those of enterohemorrhagic E. coli NleB1. The integration of these mutants into S. enterica increases S. enterica survival in macrophages, suggesting that better enzymatic kinetic parameters lead to enhanced virulence. Our findings provide insights into how these enzymes finely tune arginine-glycosylation and, in turn, bacterial virulence. In addition, our data show how promiscuous glycosyltransferases preferentially glycosylate specific protein substrates. |
Agencias financiadoras | Ministerio de Ciencia, Innovación y Universidades (MICINN). España Gobierno de Aragón Biotechnology and Biological Sciences Research Council National Institute of Allergy and Infectious Diseases (NIAID) National Institutes of Health. United States |
Identificador del proyecto | BFU2016-75633-P
PID2019-105451GB-I00 PID2019-109395GB-I00 BB/P010660/1 BB/M011216/1 E34_R17 LMP58_18 AI127973 AI153202 P20GM130448 |
Cita | García García, A., Hicks, T., El Qaidi, S., Zhu, C., Hardwidge, P.R., Angulo Álvarez, J. y Hurtado Guerrero, R. (2021). NleB/SseK-catalyzed arginine-glycosylation and enteropathogen virulence are finely tuned by a single variable position contiguous to the catalytic machinery. Chemical Science, 12 (36), 12181-12191. |
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