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dc.creatorPalanski, Brad A.es
dc.creatorWeng, Nielsones
dc.creatorZhang, Lichaoes
dc.creatorHilmer, Andrew J.es
dc.creatorFall, Lalla A.es
dc.creatorSwaminathan, Kavyaes
dc.creatorJabri, Banaes
dc.creatorSousa Martín, Carolinaes
dc.creatorFernandez-Becker, Nielsen Q.es
dc.creatorKhosla, Chaitanes
dc.creatorElias, Joshua E.es
dc.date.accessioned2022-03-01T16:16:16Z
dc.date.available2022-03-01T16:16:16Z
dc.date.issued2022
dc.identifier.citationPalanski, B.A., Weng, N., Zhang, L., Hilmer, A.J., Fall, L.A., Swaminathan, K.,...,Elias, J.E. (2022). An efficient urine peptidomics workflow identifies chemically defined dietary gluten peptides from patients with celiac disease. Nature Communications, 13 (1), 888.
dc.identifier.issn2041-1723es
dc.identifier.urihttps://hdl.handle.net/11441/130291
dc.description.abstractCeliac disease (CeD) is an autoimmune disorder induced by consuming gluten proteins from wheat, barley, and rye. Glutens resist gastrointestinal proteolysis, resulting in peptides that elicit inflammation in patients with CeD. Despite well-established connections between glutens and CeD, chemically defined, bioavailable peptides produced from dietary proteins have never been identified from humans in an unbiased manner. This is largely attributable to technical challenges, impeding our knowledge of potentially diverse peptide species that encounter the immune system. Here, we develop a liquid chromatographic-mass spectrometric workflow for untargeted sequence analysis of the urinary peptidome. We detect over 600 distinct dietary peptides, of which ~35% have a CeD-relevant T cell epitope and ~5% are known to stimulate innate immune responses. Remarkably, gluten peptides from patients with CeD qualitatively and quantitatively differ from controls. Our results provide a new foundation for understanding gluten immunogenicity, improving CeD management, and characterizing the dietary and urinary peptidomes.es
dc.description.sponsorshipMinisterio de Ciencia e Innovación SAF2017-83700-Res
dc.formatapplication/pdfes
dc.format.extent13 p.es
dc.language.isoenges
dc.publisherSpringer Naturees
dc.relation.ispartofNature Communications, 13 (1), 888.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleAn efficient urine peptidomics workflow identifies chemically defined dietary gluten peptides from patients with celiac diseasees
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiología y Parasitologíaes
dc.relation.projectIDSAF2017-83700-Res
dc.relation.publisherversionhttps://doi.org/10.1038/s41467-022-28353-1es
dc.identifier.doi10.1038/s41467-022-28353-1es
dc.journaltitleNature Communicationses
dc.publication.volumen13es
dc.publication.issue1es
dc.publication.initialPage888es

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