dc.creator | Gómez-González, Emilio | es |
dc.creator | Barriga-Rivera, Alejandro | es |
dc.creator | Fernández Muñoz, Beatriz | es |
dc.creator | Navas-García, José Manuel | es |
dc.creator | Fernández-Lizaranzu, Isabel | es |
dc.creator | Muñoz-González, Francisco Javier | es |
dc.date.accessioned | 2022-02-24T13:16:32Z | |
dc.date.available | 2022-02-24T13:16:32Z | |
dc.date.issued | 2022-02 | |
dc.identifier.citation | Gómez-González, E., Barriga-Rivera, A., Fernández-Muñoz, B., Navas-García, J.M., Fernández-Lizaranzu, I. , Muñoz-González, F.J. [et al.] (2022). Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept. Scientific Reports, 12, 2356. | |
dc.identifier.issn | 2045-2322 | es |
dc.identifier.uri | https://hdl.handle.net/11441/130208 | |
dc.description.abstract | Effective testing is essential to control the coronavirus disease 2019 (COVID-19) transmission. Here we report a-proof-of-concept study on hyperspectral image analysis in the visible and near-infrared range for primary screening at the point-of-care of SARS-CoV-2. We apply spectral feature descriptors, partial least square-discriminant analysis, and artificial intelligence to extract information from optical diffuse reflectance measurements from 5 µL fluid samples at pixel, droplet, and patient levels. We discern preparations of engineered lentiviral particles pseudotyped with the spike protein of the SARS-CoV-2 from those with the G protein of the vesicular stomatitis virus in saline solution and artificial saliva. We report a quantitative analysis of 72 samples of nasopharyngeal exudate in a range of SARS-CoV-2 viral loads, and a descriptive study of another 32 fresh human saliva samples. Sensitivity for classification of exudates was 100% with peak specificity of 87.5% for discernment from PCR-negative but symptomatic cases. Proposed technology is reagent-free, fast, and scalable, and could substantially reduce the number of molecular tests currently required for COVID-19 mass screening strategies even in resource-limited settings. | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación, 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease Instituto de Salud Carlos III by Grant Number EQC2019-006240-P | es |
dc.description.sponsorship | Comisión Europea, Joint Research Center (JRC) HUMAINT project. | es |
dc.format | application/pdf | es |
dc.format.extent | 18 p. | es |
dc.language.iso | eng | es |
dc.publisher | Nature Portfolio | es |
dc.relation.ispartof | Scientific Reports, 12, 2356. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Optical imaging spectroscopy for rapid, primary screening of SARS-CoV-2: a proof of concept | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Física Aplicada III | es |
dc.relation.projectID | COV20-00080 | es |
dc.relation.projectID | COV20-00173 | es |
dc.relation.projectID | MICIN/AEI/10.13039/501100011033 | es |
dc.relation.publisherversion | https://www.nature.com/articles/s41598-022-06393-3 | es |
dc.identifier.doi | 10.1038/s41598-022-06393-3 | es |
dc.journaltitle | Scientific Reports | es |
dc.publication.volumen | 12 | es |
dc.publication.initialPage | 2356 | es |