dc.creator | Díaz Moreno, Irene | es |
dc.creator | Díaz Quintana, Antonio Jesús | es |
dc.creator | Molina Heredia, Fernando Publio | es |
dc.creator | Nieto Mesa, Pedro Manuel | es |
dc.creator | Hansson, Örjan | es |
dc.creator | Rosa Acosta, Miguel Ángel de la | es |
dc.creator | Karlsson, B. Göran | |
dc.date.accessioned | 2022-02-17T15:49:32Z | |
dc.date.available | 2022-02-17T15:49:32Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Díaz Moreno, I., Díaz Quintana, A.J., Molina Heredia, F.P., Nieto Mesa, P.M., Hansson, Ö. y Rosa Acosta, M.Á.d.l. Karlsson, B. G. (2005). NMR analysis of the transient complex between membrane photosystem I and soluble cytochrome c6. Journal of Biological Chemistry, 280 (9), 7925-7931. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | https://hdl.handle.net/11441/130049 | |
dc.description.abstract | A structural analysis of the surface areas of cytochrome c6, responsible for the transient interaction with photosystem I, was performed by NMR transverse relaxation-optimized spectroscopy. The hemeprotein was titrated by adding increasing amounts of the chlorophyllic photosystem, and the NMR spectra of the free and bound protein were analyzed in a comparative way. The NMR signals of cytochrome c6 residues located at the hydrophobic and electrostatic patches, which both surround the heme cleft, were specifically modified by binding. The backbones of internal residues close to the hydrophobic patch of cytochrome c6 were also affected, a fact that is ascribed to the conformational changes taking place inside the hemeprotein when interacting with photosystem I. To the best of our knowledge, this is the first structural analysis by NMR spectroscopy of a transient complex between soluble and membrane proteins. | es |
dc.description.sponsorship | European Commission CT1999-00095 | es |
dc.description.sponsorship | Ministerio de Educación, Cultura y Deporte AP2000-2937 | es |
dc.description.sponsorship | Ministerio de Ciencia y Tecnología BMC2003-00458 | es |
dc.description.sponsorship | Junta de Andalucía CVI0198 | es |
dc.format | application/pdf | es |
dc.format.extent | 7 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Biological Chemistry, 280 (9), 7925-7931. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | NMR analysis of the transient complex between membrane photosystem I and soluble cytochrome c6 | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | CT1999-00095 | es |
dc.relation.projectID | AP2000-2937 | es |
dc.relation.projectID | BMC2003-00458 | es |
dc.relation.projectID | CVI0198 | es |
dc.relation.publisherversion | https://doi.org/10.1074/jbc.M412422200 | es |
dc.identifier.doi | 10.1074/jbc.M412422200 | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 280 | es |
dc.publication.issue | 9 | es |
dc.publication.initialPage | 7925 | es |
dc.publication.endPage | 7931 | es |