dc.creator | Serrano Bueno, Gloria | es |
dc.creator | Hernández, Agustín | es |
dc.creator | López Lluch, Guillermo | es |
dc.creator | Pérez Castiñeira, José Román | es |
dc.creator | Navas, Plácido | es |
dc.creator | Serrano Delgado, Aurelio | es |
dc.date.accessioned | 2022-02-08T17:15:37Z | |
dc.date.available | 2022-02-08T17:15:37Z | |
dc.date.issued | 2013 | |
dc.identifier.citation | Serrano Bueno, G., Hernández, A., López Lluch, G., Pérez Castiñeira, J.R., Navas, P. y Serrano Delgado, A. (2013). Inorganic pyrophosphatase defects lead to cell cycle arrest and autophagic cell death through NAD+ depletion in fermenting yeast. Journal of Biological Chemistry, 288 (18), 13082-13092. | |
dc.identifier.issn | 0021-9258 | es |
dc.identifier.issn | 1083-351X | es |
dc.identifier.uri | https://hdl.handle.net/11441/129780 | |
dc.description.abstract | Inorganic pyrophosphatases are required for anabolism to take place in all living organisms. Defects in genes encoding these hydrolytic enzymes are considered inviable, although their exact nature has not been studied at the cellular and molecular physiology levels. Using a conditional mutant in IPP1, the Saccharomyces cerevisiae gene encoding the cytosolic soluble pyrophosphatase,we show that respiring cells arrest in S phase upon Ipp1p deficiency, but they remain viable and resume growth if accumulated pyrophosphate is removed. However, fermenting cells arrest in G 1/G0 phase and suffer massive vacuolization and eventual cell death by autophagy. Impaired NAD+ metabolism is a major determinant of cell death in this scenario because demise can be avoided under conditions favoring accumulation of the oxidized pyridine coenzyme. These results posit that the mechanisms related to excess pyrophosphate toxicity in eukaryotes are dependent on the energy metabolism of the cell. | es |
dc.description.sponsorship | Junta de Andalucía P07-CVI-3082 | es |
dc.description.sponsorship | Ministerio de Ciencia e Innovación BFU2007-61887, BFU2010-15622 | es |
dc.description.sponsorship | Ministerio de Sanidad, Política Social e Igualdad FIS-PI080500, P08-CTS-3988 | es |
dc.format | application/pdf | es |
dc.format.extent | 11 p. | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Journal of Biological Chemistry, 288 (18), 13082-13092. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | Inorganic pyrophosphatase defects lead to cell cycle arrest and autophagic cell death through NAD+ depletion in fermenting yeast | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | P07-CVI-3082 | es |
dc.relation.projectID | BFU2007-61887 | es |
dc.relation.projectID | BFU2010-15622 | es |
dc.relation.projectID | FIS-PI080500 | es |
dc.relation.projectID | P08-CTS-3988 | es |
dc.relation.publisherversion | https://doi.org/10.1074/jbc.M112.439349 | es |
dc.identifier.doi | 10.1074/jbc.M112.439349 | es |
dc.journaltitle | Journal of Biological Chemistry | es |
dc.publication.volumen | 288 | es |
dc.publication.issue | 18 | es |
dc.publication.initialPage | 13082 | es |
dc.publication.endPage | 13092 | es |