dc.creator | Castell Capitán, María Carmen | es |
dc.creator | Bernal Bayard, Pilar | es |
dc.creator | Ortega Rodríguez, José María | es |
dc.creator | Roncel Gil, Mercedes | es |
dc.creator | Hervás Morón, Manuel | es |
dc.creator | Navarro Carruesco, José Antonio | es |
dc.date.accessioned | 2021-09-22T17:01:08Z | |
dc.date.available | 2021-09-22T17:01:08Z | |
dc.date.issued | 2021 | |
dc.identifier.citation | Castell Capitán, M.C., Bernal Bayard, P., Ortega Rodríguez, J.M., Roncel Gil, M., Hervás Morón, M. y Navarro Carruesco, J.A. (2021). The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions. Physiologia Plantarum, 171 (2), 277-290. | |
dc.identifier.issn | 0031-9317 | es |
dc.identifier.issn | 1399-3054 | es |
dc.identifier.uri | https://hdl.handle.net/11441/126118 | |
dc.description.abstract | We have investigated if the heterologous expression of a functional green alga plastocyanin in the diatom Phaeodactylum tricornutum can improve photosynthetic activity and cell growth. Previous in vitro assays showed that a single-mutant of the plastocyanin from the green algae Chlamydomonas reinhardtii is effective in reducing P. tricornutum photosystem I. In this study, in vivo assays with P. tricornutum strains expressing this plastocyanin indicate that even the relatively low intracellular concentrations of holo-plastocyanin detected (≈4 μM) are enough to promote an increased growth (up to 60%) under iron-deficient conditions as compared with the WT strain, measured as higher cell densities, content in pigments and active photosystem I, global photosynthetic rates per cell, and even cell volume. In addition, the presence of plastocyanin as an additional photosynthetic electron carrier seems to decrease the over-reduction of the plastoquinone pool. Consequently, it promotes an improvement in the maximum quantum yield of both photosystem II and I, together with a decrease in the acceptor side photoinhibition of photosystem II—also associated to a reduced oxidative stress—a decrease in the peroxidation of membrane lipids in the choroplast, and a lower degree of limitation on the donor side of photosystem I. Thus the heterologous plastocyanin appears to act as a functional electron carrier, alternative to the native cytochrome c6, under iron-limiting conditions. | es |
dc.description.sponsorship | Junta de Andalucía PAIDI BIO-022 | es |
dc.description.sponsorship | Ministerio de Economía y Competitividad BIO2015-64169-P | es |
dc.format | application/pdf | es |
dc.format.extent | 14 p. | es |
dc.language.iso | eng | es |
dc.publisher | Wiley-Blackwell | es |
dc.relation.ispartof | Physiologia Plantarum, 171 (2), 277-290. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The heterologous expression of a plastocyanin in the diatom Phaeodactylum tricornutum improves cell growth under iron-deficient conditions | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | PAIDI BIO-022 | es |
dc.relation.projectID | BIO2015-64169-P | es |
dc.relation.publisherversion | https://doi.org/10.1111/ppl.13290 | es |
dc.identifier.doi | 10.1111/ppl.13290 | es |
dc.journaltitle | Physiologia Plantarum | es |
dc.publication.volumen | 171 | es |
dc.publication.issue | 2 | es |
dc.publication.initialPage | 277 | es |
dc.publication.endPage | 290 | es |