Artículo
Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents
Autor/es | Moyá Morán, María Luisa
Ostos Marcos, Francisco José Moreno, Izamar García, Diandra Moreno Gordillo, Paula Valle Rosado, Iván López-Cornejo, María del Pilar Lebrón Romero, José Antonio López López, Manuel |
Departamento | Universidad de Sevilla. Departamento de Química Física |
Fecha de publicación | 2021 |
Fecha de depósito | 2021-07-05 |
Publicado en |
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Resumen | The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, ... The obtaining of nanocarriers of gene material and small drugs is still an interesting research line. Side-effects produced by the toxicity of several pharmaceutics, the high concentrations needed to get therapeutic effects, or their excessive use by patients have motivated the search for new nanostructures. For these reasons, cationic metallo-liposomes composed by phosphatidylcholine (PC), cholesterol (CHO) and RuC1C19 (a surfactant derived from the metallic complex [Ru(bpy)3]2+) were prepared and characterized by using diverse techniques (zeta potential, dynamic light scattering and electronic transmission microscopy –TEM-). Unimodal or bimodal populations of spherical aggregates with small sizes were obtained depending on the composition of the liposomes. The presence of cholesterol favored the formation of small aggregates. ct-DNA was condensed in the presence of the liposomes investigated. In-vitro assays demonstrated the ability of these nanoaggregates to internalize into different cell lines. A positive gene transfection into human bone osteosarcoma epithelial cells (U2OS) was also observed. The RuC1C19 surfactant was used as sensor to quantify the binding of DNA to the liposomes. Doxorubicin was encapsulated into the metallo-liposomes, demonstrating their ability to be also used as nanocarriers of drugs. A relationship between then encapsulation percentage of the antibiotic and the composition of the aggregates has been established. |
Identificador del proyecto | 2019/FQM-206
2019/FQM-274 RTI2018-100692-BI00 PI-0005-2018 P18-RT-1271 |
Cita | Moyá Morán, M.L., Ostos Marcos, F.J., Moreno, I., García, D., Moreno Gordillo, P., Valle Rosado, I.,...,López López, M. (2021). Metallo-Liposomes Derived from the [Ru(bpy)3]2+ Complex as Nanocarriers of Therapeutic Agents. Chemosensors, 9 (5), 90. |
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