Artículo
Biochemical characterization of the amylase activity from the new haloarchaeal strain haloarcula sp. Hs isolated in the odiel marshlands
Autor/es | Gómez Villegas, Patricia
Vigara, Javier Romero González, Luis Carlos Gotor Martínez, Cecilia Raposo, Sara Gonçalves, Brígida León Bañares, Rosa María |
Departamento | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Fecha de publicación | 2021 |
Fecha de depósito | 2021-05-20 |
Publicado en |
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Resumen | Alpha‐amylases are a large family of α,1‐4‐endo‐glycosyl hydrolases distributed in all kingdoms of life. The need for poly‐extremotolerant amylases encouraged their search in extreme environments, where archaea become ideal ... Alpha‐amylases are a large family of α,1‐4‐endo‐glycosyl hydrolases distributed in all kingdoms of life. The need for poly‐extremotolerant amylases encouraged their search in extreme environments, where archaea become ideal candidates to provide new enzymes that are able to work in the harsh conditions demanded in many industrial applications. In this study, a collection of haloarchaea isolated from Odiel saltern ponds in the southwest of Spain was screened for their amylase activity. The strain that exhibited the highest activity was selected and identified as Halo-arcula sp. HS. We demonstrated the existence in both, cellular and extracellular extracts of the new strain, of functional α‐amylase activities, which showed to be moderately thermotolerant (optimum around 60 °C), extremely halotolerant (optimum over 25% NaCl), and calcium‐dependent. The tryp-tic digestion followed by HPLC‐MS/MS analysis of the partially purified cellular and extracellular extracts allowed to identify the sequence of three alpha‐amylases, which despite sharing a low sequence identity, exhibited high three‐dimensional structure homology, conserving the typical do-mains and most of the key consensus residues of α‐amylases. Moreover, we proved the potential of the extracellular α‐amylase from Haloarcula sp. HS to treat bakery wastes under high salinity con-ditions. |
Agencias financiadoras | Agencia Estatal de Investigación. España PID2019-109785GB-I00, PID2019-110438RB-C22 -AEI/FEDER |
Identificador del proyecto | PID2019-109785GB-I00
PID2019-110438RB-C22 -AEI/FEDER P18-RT-3154 |
Cita | Gómez Villegas, P., Vigara, J., Romero González, L.C., Gotor Martínez, C., Raposo, S., Gonçalves, B. y León Bañares, R.M. (2021). Biochemical characterization of the amylase activity from the new haloarchaeal strain haloarcula sp. Hs isolated in the odiel marshlands. Biology, 10 (4), 337. |
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