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Artículo
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Flow Cytometry of Microencapsulated Colonies for Genetics Analysis of Filamentous Fungi
(Genetics Society of America:, 2014)
The analysis of filamentous fungi by flow cytometry has been impossible to date due to their filamentous nature and size. In this work, we have developed a method that combines single-spore microencapsulation and large-particle ...
Artículo
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FACT Prevents the Accumulation of Free Histones Evicted from Transcribed Chromatin and a Subsequent Cell Cycle Delay in G1
(Public Library of Science, 2010)
The FACT complex participates in chromatin assembly and disassembly during transcription elongation. The yeast mutants affected in the SPT16 gene, which encodes one of the FACT subunits, alter the expression of G1 cyclins ...
Artículo
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Single cell analysis of bistable expression of pathogenicity island 1 and the flagellar regulon in Salmonella enterica
(MDPI, 2021-01)
Bistable expression of the Salmonella enterica pathogenicity island 1 (SPI-1) and the flagellar network (Flag) has been described previously. In this study, simultaneous monitoring of OFF and ON states in SPI-1 and in the ...
Tesis Doctoral
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Identificación y caracterización de elementos regulatorios distales controlados por TGFβ
(2021-03-02)
En este trabajo se ha realizado un análisis exhaustivo de los enhancers en células normales de epitelio de glándula mamaria de ratón (NMuMG) para entender, de forma más detallada, cómo los enhancers dependientes de TGFβ ...
Tesis Doctoral
Tesis Doctoral
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Contribución del regulon Rcs a la virulencia de Salmonella análisis genético y molecular
(2008)
En el momento de iniciar esta tesis, trabajos recientes de nuestro laboratorio en colaboración con el Dr. García del Portillo habían descrito que alelos viables del gen esencial igaA activaban el sistema de transducción ...
Artículo
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Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system
(National Academy of Sciences, 2015)
DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset ...
Tesis Doctoral
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Entrada y salida de treonina y homoserina en Saccharomyces cerevisiae mediada por transportadores de aminoácidos
(2005-10-07)
La excreción de matabolitos, tales como aminoácidos, por la levadura S. cerevisiae es un fenómeno documentado desde hace al menos cuatro décadas (Grenson, 1973; Lewis y Phaff, 1964). Las investigaciones realizada a este ...
Artículo
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Genome-Wide Identification and Expression Analysis of SOS Response Genes in Salmonella enterica Serovar Typhimurium
(Multidisciplinary Digital Publishing Institute (MDPI), 2021)
A bioinformatic search for LexA boxes, combined with transcriptomic detection of loci responsive to DNA damage, identified 48 members of the SOS regulon in the genome of Salmonella enterica serovar Typhimurium. Single cell ...
Artículo
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Tubulin Folding Cofactor TBCB is a Target of the Salmonella Effector Protein SseK1
(MDPI, 2020)
Salmonella enterica serovar Typhimurium is a human and animal pathogen that uses type III secretion system effectors to manipulate the host cell and fulfill infection. SseK1 is a Salmonella effector with glycosyltransferase ...