dc.creator | Jurado Flores, Ana | es |
dc.creator | Delgado Requerey, Victor | es |
dc.creator | Gálvez Ramírez, Alicia | es |
dc.creator | Puerto Galán, Leonor | es |
dc.creator | Pérez Ruiz, Juan Manuel | es |
dc.creator | Cejudo Fernández, Francisco Javier | es |
dc.date.accessioned | 2020-11-19T13:19:11Z | |
dc.date.available | 2020-11-19T13:19:11Z | |
dc.date.issued | 2020 | |
dc.identifier.citation | Jurado Flores, A., Delgado Requerey, V., Gálvez Ramírez, A., Puerto Galán, L., Pérez Ruiz, J.M. y Cejudo Fernández, F.J. (2020). Exploring the Functional Relationship between y-Type Thioredoxins and 2-Cys Peroxiredoxins in Arabidopsis Chloroplasts. Antioxidants, 9 (11), 1-18. | |
dc.identifier.issn | 2076-3921 | es |
dc.identifier.uri | https://hdl.handle.net/11441/102731 | |
dc.description.abstract | Thioredoxins (Trxs) are small, ubiquitous enzymes that catalyze disulphide–dithiol
interchange in target enzymes. The large set of chloroplast Trxs, including f, m, x and y subtypes,
use reducing equivalents fueled by photoreduced ferredoxin (Fdx) for fine-tuning photosynthetic
performance and metabolism through the control of the activity of redox-sensitive proteins.
Although biochemical analyses suggested functional diversity of chloroplast Trxs, genetic studies
have established that deficiency in a particular Trx subtype has subtle phenotypic e ects, leading to
the proposal that the Trx isoforms are functionally redundant. In addition, chloroplasts contain
an NADPH-dependent Trx reductase with a joint Trx domain, termed NTRC. Interestingly,
Arabidopsis mutants combining the deficiencies of x- or f-type Trxs and NTRC display very severe
growth inhibition phenotypes, which are partially rescued by decreased levels of 2-Cys peroxiredoxins
(Prxs). These findings indicate that the reducing capacity of Trxs f and x is modulated by the redox
balance of 2-Cys Prxs, which is controlled by NTRC. In this study, we explored whether NTRC acts
as a master regulator of the pool of chloroplast Trxs by analyzing its functional relationship with
Trxs y. While Trx y interacts with 2-Cys Prxs in vitro and in planta, the analysis of Arabidopsis mutants
devoid of NTRC and Trxs y suggests that Trxs y have only a minor e ect, if any, on the redox state of
2-Cys Prxs. | es |
dc.format | application/pdf | es |
dc.format.extent | 18 p. | es |
dc.language.iso | eng | es |
dc.publisher | MDPI | es |
dc.relation.ispartof | Antioxidants, 9 (11), 1-18. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | 2-Cys peroxiredoxin | es |
dc.subject | Thioredoxin y | es |
dc.subject | NTRC | es |
dc.subject | Thioredoxin x | es |
dc.subject | Redox regulation | es |
dc.subject | Stress response | es |
dc.subject | Photosynthesis | es |
dc.subject | Chloroplast | es |
dc.title | Exploring the Functional Relationship between y-Type Thioredoxins and 2-Cys Peroxiredoxins in Arabidopsis Chloroplasts | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.publisherversion | https://doi.org/10.3390/antiox9111072 | es |
dc.identifier.doi | 10.3390/antiox9111072 | es |
dc.journaltitle | Antioxidants | es |
dc.publication.volumen | 9 | es |
dc.publication.issue | 11 | es |
dc.publication.initialPage | 1 | es |
dc.publication.endPage | 18 | es |