Artículo
Brain Organoids to Evaluate Cellular Therapies
Autor/es | García Delgado, Ana Belén
Campos Cuerva, Rafael Rosell Valle, Cristina Martín López, María Casado, Carlos Ferrari, Daniela Sánchez Pernaute, Rosario Fernández Muñoz, Beatriz Márquez-Rivas, Javier |
Departamento | Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica Universidad de Sevilla. Departamento de Cirugía |
Fecha de publicación | 2022 |
Fecha de depósito | 2023-05-23 |
Publicado en |
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Resumen | Animal models currently used to test the efficacy and safety of cell therapies, mainly murine models, have limitations as molecular, cellular, and physiological mechanisms are often inherently different between species, ... Animal models currently used to test the efficacy and safety of cell therapies, mainly murine models, have limitations as molecular, cellular, and physiological mechanisms are often inherently different between species, especially in the brain. Therefore, for clinical translation of cell-based medicinal products, the development of alternative models based on human neural cells may be crucial. We have developed an in vitro model of transplantation into human brain organoids to study the potential of neural stem cells as cell therapeutics and compared these data with standard xenograft studies in the brain of immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice. Neural stem cells showed similar differentiation and proliferation potentials in both human brain organoids and mouse brains. Our results suggest that brain organoids can be informative in the evaluation of cell therapies, helping to reduce the number of animals used for regulatory studies. |
Agencias financiadoras | Instituto de Salud Carlos III |
Identificador del proyecto | DTS20/00108
PT20/00065 |
Cita | García Delgado, A.B., Campos Cuerva, R., Rosell Valle, C., Martín López, M., Casado, C., Ferrari, D.,...,Márquez-Rivas, J. (2022). Brain Organoids to Evaluate Cellular Therapies. Animals, 12 (22). https://doi.org/10.3390/ani12223150. |
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