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Browsing by Author "Borrero de Acuña, José Manuel"
Now showing items 1-13 of 13
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Article
A Point Mutation in the Transcriptional Repressor PerR Results in a Constitutive Oxidative Stress Response in Clostridioides difficile 630(delta)Derm
Troitzsch, D; Zhang, H; Dittmann, S; Dusterhoft, D; Moller, TA; Borrero de Acuña, José Manuel; Sievers, S (American Society for Microbiology, 2021-03-03)The human pathogen Clostridioides difficile has evolved into the leading cause of nosocomial diarrhea. The bacterium is ...
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Cellular adaptation of Clostridioides difficile to high salinity encompasses a compatible solute-responsive change in cell morphology
Michel, Annika Marisa; Borrero de Acuña, José Manuel; Molinari, Gabriella; Unal, Can Murat; Will, Sabine; Derksen, Elisabeth (WILEY, 2022)Infections by the pathogenic gut bacterium Clostridioides difficile cause severe diarrhoeas up to a toxic megacolon and ...
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Channelling carbon flux through the meta-cleavage route for improved poly(3-hydroxyalkanoate) production from benzoate and lignin-based aromatics in Pseudomonas putida H
Borrero de Acuña, José Manuel; Gutiérrez Urrutia, Izabook; Hidalgo Dumont, Cristian; Aravena Carrasco, Carla; Orellana Saez, Matias; Palominos González, Nestor (Wiley Open Access, 2020-11-10)Lignin-based aromatics are attractive raw materials to derive medium-chain length poly(3-hydroxyalkanoates) (mcl-PHAs), ...
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Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)
Poblete-Castro, Ignacio; Aravena-Carrasco, Carla; Orellana-Sáez, Matías; Pacheco, Nicolás; Cabrera, Álex; Borrero de Acuña, José Manuel (Frontiers Research Foundation, 2020)In the last decade, the development of novel programmable cell lytic systems based on different inducible genetic constructs ...
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Fed-Batch mcl- Polyhydroxyalkanoates Production in Pseudomonas putida KT2440 and ΔphaZ Mutant on Biodiesel-Derived Crude Glycerol
Borrero de Acuña, José Manuel; Rohde, Manfred; Saldias, Cesar; Poblete Castro, Ignacio (Frontiers Research Foundation, 2021-03-16)Crude glycerol has emerged as a suitable feedstock for the biotechnological production of various industrial chemicals ...
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Isolation and Quantification of Bacterial Membrane Vesicles for Quantitative Metabolic Studies Using Mammalian Cell Cultures
Kretschmer, Marcel; Müller, Julia; Henke, Petra; Otto, Viktoria; Arce Rodríguez, Alejandro; Müsken, Mathias; Jahn, Dieter; Borrero de Acuña, José Manuel; Neumann-Schaal, Meina; Wegner, Andre (Multidisciplinary Digital Publishing Institute (MDPI), 2023-11-21)Bacterial membrane vesicles (BMVs) are produced by most bacteria and participate in various cellular processes, such as ...
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Article
Legionella pneumophila PPIase Mip Interacts with the Bacterial Proteins SspB, Lpc2061, and FlaA and Promotes Flagellation
Karagöz, Mustafa Safa; Ünal, Can Murat; Mayer, Benjamin E.; Müsken, Mathias; Borrero de Acuña, José Manuel; Steinert, Michael (American Society for Microbiology, 2022-10-31)The peptidyl-prolyl-cis/trans-isomerase (PPIase) macrophage infectivity potentiator (Mip) contributes to the pathogenicity ...
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Membrane vesicle engineering with “à la carte” bacterial-immunogenic molecules for organism-free plant vaccination
Jiménez Guerrero, Irene; López Baena, Francisco Javier; Borrero de Acuña, José Manuel; Pérez Montaño, Francisco de Asís (Wiley-Blackwell, 2023)The United Nations heralds a world population exponential increase exceeding 9.7 billion by 2050. This poses the challenge ...
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Plant holobiont interactions mediated by the type VI secretion system and the membrane vesicles: promising tools for a greener agriculture
Borrero de Acuña, José Manuel; Bernal Guzmán, Patricia (Wiley, 2021)A deeper understanding of the complex relationship between plants and their microbiota is allowing researchers to appreciate ...
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Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery
Borrero de Acuña, José Manuel; Poblete Castro, Ignacio (Wiley-Blackwell, 2023-02)Microbial production of biopolymers derived from renewable substrates and waste streams reduces our heavy reliance on ...
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Standardization of inducer-activated broad host range expression modules: debugging and refactoring an alkane-responsive AlkS/PalkB device
Arce Rodríguez, Alejandro; Benedetti, Ilaria; Borrero de Acuña, José Manuel; Silva Rocha, Rafael; Lorenzo, Víctor de (Oxford University Press, 2021-09-17)Although inducible heterologous expression systems have been available since the birth of recombinant DNA technology, the ...
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Article
The alternative coproporphyrinogen III oxidase (CgoN) catalyzes the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III
Mingers, Toni; Barthels, Stefan; Mass, Violetta; Borrero de Acuña, José Manuel; Biedendieck, Rebekka; Cooke, Ana; Dailey, Tamara A.; Gerdes, Svetlana; Blankenfeldt, Wulf; Dailey, Harry A.; Warren, Martin J.; Jahn, Martina; Jahn, Dieter (Frontiers Media S.A., 2024-03-13)Nature utilizes three distinct pathways to synthesize the essential enzyme cofactor heme. The coproporphyrin III-dependent ...
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Towards Translation of PqsR Inverse Agonists: From In Vitro Efficacy Optimization to In Vivo Proof-of-Principle
Hamed, Mostafa M.; Abdelsamie, Ahmed S.; Rox, Katharina; Schütz, Christian; Kany, Andreas M.; Röhrig, Teresa; Schmelz, Stefan; Blankenfeldt, Wulf; Arce Rodríguez, Alejandro; Borrero de Acuña, José Manuel; Empting, Martin (Wiley-Blackwell, 2023-02-14)Pseudomonas aeruginosa (PA) is an opportunistic human pathogen, which is involved in a wide range of dangerous infections. ...