Artículos (Bioquímica Vegetal y Biología Molecular)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/10824

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Exopolysaccharide Production by Sinorhizobium fredii HH103 Is Repressed by Genistein in a NodD1-Dependent Manner
(Public Library of Science, 2016) Acosta Jurado, Sebastián; Navarro Gómez, Pilar; Murdoch, Piedad del Socorro; Crespo Rivas, Juan Carlos; Shi Jie; Cuesta Berrio, Lidia; Ruiz Sainz, José Enrique; Rodríguez Carvajal, Miguel Ángel; Vinardell González, José María; Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular; Universidad de Sevilla. Departamento de Microbiología; Universidad de Sevilla. Departamento de Química orgánica; Ministerio de Ciencia e Innovación (MICIN). España; Junta de Andalucía
In the rhizobia-legume symbiotic interaction, bacterial surface polysaccharides, such as exopolysaccharide (EPS), lipopolysaccharide (LPS), K-antigen polysaccharide (KPS) or cyclic glucans (CG), appear to play crucial roles either acting as signals required for the progression of the interaction and/or preventing host defence mechanisms. The symbiotic significance of each of these polysaccharides varies depending on the specific rhizobialegume couple. In this work we show that the production of exopolysaccharide by Sinorhizobium fredii HH103, but not by other S. fredii strains such as USDA257 or NGR234, is repressed by nod gene inducing flavonoids such as genistein and that this repression is dependent on the presence of a functional NodD1 protein. In agreement with the importance of EPS for bacterial biofilms, this reduced EPS production upon treatment with flavonoids correlates with decreased biofilm formation ability. By using quantitative RT-PCR analysis we show that expression of the exoY2 and exoK genes is repressed in late stationary cultures of S. fredii HH103 upon treatment with genistein. Results presented in this work show that in S. fredii HH103 EPS production is regulated just in the opposite way than other bacterial signals such as Nod factors and type 3 secreted effectors: it is repressed by flavonoids and NodD1 and enhanced by the nod repressor NolR. These results are in agreement with our previous observations showing that lack of EPS production by S. fredii HH103 is not only non-detrimental but even beneficial for symbiosis with soybean
The Sinorhizobium fredii HH103 Lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules
(2013) Fedorova, Elena; Buendía Clavería, Ana María; Rodríguez Navarro, Dulce Nombre; Ruiz Sainz, José Enrique; Vinardell González, José María; Rodríguez Carvajal, Miguel Ángel; Hidalgo Perea, Ángeles; Margaret Oliver, Isabel María; Acosta Jurado, Sebastián; Mercedes Luc; Universidad de Sevilla. Departamento de Microbiología; Universidad de Sevilla. Departamento de Química Orgánica; Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular

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Examinando Artículos (Bioquímica Vegetal y Biología Molecular) por Autor "Acosta Jurado, Sebastián"