Artículos (Instituto de Bioquímica Vegetal y Fotosíntesis IBVF – CIC Cartuja)

URI permanente para esta colecciónhttps://hdl.handle.net/11441/10949

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Examinando Artículos (Instituto de Bioquímica Vegetal y Fotosíntesis IBVF – CIC Cartuja) por Agencia financiadora "Carl Tryggers Foundation"

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    An Actin Remodeling Role for Arabidopsis Processing Bodies Revealed by their Proximity Interactome
    (WILEY, 2023) Liu, Chen; Mentzelopoulou, Andriani; Muhammad, Amna; Volkov, Andriy; Weijers, Dolf; Gutiérrez Beltrán, Emilio; Moschou, Panagiotis N.; Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular; Carl Tryggers Foundation; European Union (UE); Vetenskapsradet (VR) research council; FORMAS research council; Helge Ax:son Johnsons stiftelse; Hellenic Foundation of Research and Innovation (HFRI); European Research Council (ERC); Ministerio de Ciencia e Innovación (MICIN). España
    Cellular condensates can comprise membrane-less ribonucleoprotein assemblies with liquid-like properties. These cellular condensates influence various biological outcomes, but their liquidity hampers their isolation and characterization. Here, we investigated the composition of the condensates known as processing bodies (PBs) in the model plant Arabidopsis thaliana through a proximity-biotinylation proteomics approach. Using in situ protein–protein interaction approaches, genetics and high-resolution dynamic imaging, we show that processing bodies comprise networks that interface with membranes. Surprisingly, the conserved component of PBs, DECAPPING PROTEIN 1 (DCP1), can localize to unique plasma membrane subdomains including cell edges and vertices. We characterized these plasma membrane interfaces and discovered a developmental module that can control cell shape. This module is regulated by DCP1, independently from its role in decapping, and the actin-nucleating SCAR–WAVE complex, whereby the DCP1–SCAR–WAVE interaction confines and enhances actin nucleation. This study reveals an unexpected function for a conserved condensate at unique membrane interfaces.
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    Acceso AbiertoArtículo
    Interactome of Arabidopsis ATG5 Suggests Functions beyond Autophagy
    (Multidisciplinary Digital Publishing Institute (MDPI), 2023) Elander, Pernilla H.; Holla, Sanjana; Sabljic, Igor; Gutiérrez Beltrán, Emilio; Willems, Patrick; Bozhkov, Peter V.; Minina, Elena A.; Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular; Swedish Research Council Formas. Sweden; Carl Tryggers Foundation; Ministerio de Ciencia e Innovación (MICIN). España; Junta de Andalucía
    Autophagy is a catabolic pathway capable of degrading cellular components ranging from individual molecules to organelles. Autophagy helps cells cope with stress by removing superfluous or hazardous material. In a previous work, we demonstrated that transcriptional upregulation of two autophagy-related genes, ATG5 and ATG7, in Arabidopsis thaliana positively affected agronomically important traits: biomass, seed yield, tolerance to pathogens and oxidative stress. Although the occurrence of these traits correlated with enhanced autophagic activity, it is possible that autophagy-independent roles of ATG5 and ATG7 also contributed to the phenotypes. In this study, we employed affinity purification and LC-MS/MS to identify the interactome of wild-type ATG5 and its autophagy-inactive substitution mutant, ATG5K128R Here we present the first interactome of plant ATG5, encompassing not only known autophagy regulators but also stress-response factors, components of the ubiquitin-proteasome system, proteins involved in endomembrane trafficking, and potential partners of the nuclear fraction of ATG5. Furthermore, we discovered post-translational modifications, such as phosphorylation and acetylation present on ATG5 complex components that are likely to play regulatory functions. These results strongly indicate that plant ATG5 complex proteins have roles beyond autophagy itself, opening avenues for further investigations on the complex roles of autophagy in plant growth and stress responses.