Article
STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments
Author/s | García Negrete, Carlos Andrés
Jiménez de Haro, María del Carmen Blasco, Javier Soto, M. Fernández Camacho, Asunción |
Publication Date | 2015 |
Deposit Date | 2018-12-03 |
Published in |
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Abstract | The methodology termed scanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) has been used in this work to study the uptake of citrate stabilized gold nanoparticles (AuNPs) (average particle ... The methodology termed scanning transmission electron microscopy in scanning electron microscopy (STEM-in-SEM) has been used in this work to study the uptake of citrate stabilized gold nanoparticles (AuNPs) (average particle sizes of 23.5 ± 4.0 nm) into tissue samples upon in vitro exposure of the dissected gills of the Ruditapes philippinarum marine bivalve to the nanoparticle suspensions. The STEM-in- SEM methodology has been optimized for achieving optimum resolution under SEM low voltage operating conditions (20–30 kV). Based on scanning microscope assessments and resolution testing (SMART), resolutions well below 10 nm were appropriately achieved by working at magnifications over 100k×, with experimental sample thickness between 300 and 200 nm. These relatively thick slices appear to be stable under the beam and help avoid NP displacement during cutting. We herein show that both localizing of the internalized nanoparticles and imaging of ultrastructural disturbances in gill tissues are strongly accessible due to the improved resolution, even at sample thicknesses higher than those normally employed in standard TEM techniques at higher voltages. Ultrastructural imaging of bio-nano features in bioaccumulation experiments have been demonstrated in this study. |
Funding agencies | Junta de Andalucía European Union (UE) |
Project ID. | PE2009-FQM-4554
PE2011-RNM-7812 TEP-217 CT-REGPOT2011-1-285895 |
Citation | García Negrete, C.A., Jiménez de Haro, M.d.C., Blasco, J., Soto, M. y Fernández Camacho, A. (2015). STEM-in-SEM high resolution imaging of gold nanoparticles and bivalve tissues in bioaccumulation experiments. Analyst, 140, 3082-3089. |
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