Article
RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions
Author/s | Cruz Gallardo, Isabel
Aroca Aguilar, Ángeles Persson, Cecilia Karlsson, B. Göran Díaz Moreno, Irene |
Publication Date | 2013 |
Deposit Date | 2018-02-14 |
Published in |
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Abstract | Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The ... Background: T-cell intracellular antigen- 1(TIA-1) is a key DNA/RNA binding protein in RNA metabolism. Results: The binding of TIA-1 RRM3 domain (isolated or fused to RRM2) to RNA is affected by pH changes. Conclusion: The pH-dependence of RRM3/RNA interaction may have a significant effect in the overall TIA-1 function. Significance: Unveiling the auxiliary role of RRM3 in RNA recognition sheds light on the TIA-1 function between cell compartments changes due to the protonation / deprotonation of TIA-1 RRM3 histidine residues. The auxiliary role of the Cterminal RRM3 domain in TIA-1 RNA recognition is poorly understood and this work provides an insight into its binding mechanisms |
Funding agencies | Junta de Andalucía European Union (UE). FP7 |
Project ID. | P07-CVI-02896
BIO198 FP7/2007-2013- 261863 |
Citation | Cruz Gallardo, I., Aroca Aguilar, Á., Persson, C., Karlsson, B.G. y Díaz Moreno, I. (2013). RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions. Journal of Biological Chemistry, 288, 25986-25994. |
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