Article
S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems
Author/s | Aroca Aguilar, Ángeles
Serna, Antonio Gotor Martínez, Cecilia Romero González, Luis Carlos |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2015 |
Deposit Date | 2018-01-18 |
Published in |
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Abstract | Hydrogen sulfide is a highly reactive molecule that is currently accepted as a signaling compound. This molecule is as important as carbon monoxide in mammals and hydrogen peroxide in plants, as well as nitric oxide in ... Hydrogen sulfide is a highly reactive molecule that is currently accepted as a signaling compound. This molecule is as important as carbon monoxide in mammals and hydrogen peroxide in plants, as well as nitric oxide in both eukaryotic systems. Although many studies have been conducted on the physiological effects of hydrogen sulfide, the underlying mechanisms are poorly understood. One of the proposed mechanisms involves the posttranslational modification of protein cysteine residues, a process called S-sulfhydration. In this work, a modified biotin switch method was used for the detection of Arabidopsis (Arabidopsis thaliana) proteins modified by S-sulfhydration under physiological conditions. The presence of an S-sulfhydration-modified cysteine residue on cytosolic ascorbate peroxidase was demonstrated using liquid chromatography-tandem mass spectrometry analysis, and a total of 106 S-sulfhydrated proteins were identified. Immunoblot and enzyme activity analyses of some of these proteins showed that the sulfide added through S-sulfhydration reversibly regulates the functions of plant proteins in a manner similar to that described in mammalian systems. |
Funding agencies | Ministerio de Economía y Competitividad (MINECO). España Junta de Andalucía |
Project ID. | grant no. BIO2013-4464 8-P
grant no. CVI-7190 |
Citation | Aroca Aguilar, Á., Serna, A., Gotor Martínez, C. y Romero González, L.C. (2015). S-Sulfhydration: A Cysteine Posttranslational Modification in Plant Systems. Plant Physiology, 168 (1), 334-342. |
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