Article
Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120
Author/s | Olmedo Verd, Elvira de
Muro Pastor, Alicia María Flores García, Enrique Herrero Moreno, Antonia |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2006 |
Deposit Date | 2017-07-24 |
Published in |
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Abstract | The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a ... The ntcA gene encodes an N-control transcriptional regulator in cyanobacteria. In the N2-fixing, heterocyst-forming cyanobacterium Anabaena sp. strain PCC 7120, ntcA is an autoregulatory gene that is transcribed from a complex promoter region that includes a constitutive promoter (P 2) and promoters that are induced upon N step-down (P1 and P3). Expression of ntcA was investigated with the use of an ntcA-gfp translational fusion, which was introduced both in the natural ntcA locus and in a heterologous genomic place. Induction of ntcA-gfp took place after N step-down in all the cells of the filament, but at especially high levels in developing heterocysts. Localized induction could be driven independently by P3 and P1. |
Funding agencies | Ministerio de Educación y Ciencia (MEC). España |
Project ID. | BFU2004-00872 |
Citation | Olmedo Verd, E., Muro Pastor, A.M., Flores García, E. y Herrero, A. (2006). Localized induction of the ntcA regulatory gene in developing heterocysts of Anabaena sp. strain PCC 7120. Journal of Bacteriology, 188 (18), 6694-6699. |
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