Artículo
Polycomb RING1A- and RING1B-dependent histone H2A monoubiquitylation at pericentromeric regions promotes S-phase progression
Autor/es | Bravo, M.
Nicolini, F. Starowicz, K. Barroso Ceballos, Sonia Inés Calés, C. Aguilera López, Andrés Vidal, M. |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2015 |
Fecha de depósito | 2017-06-28 |
Publicado en |
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Resumen | The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, ... The functions of polycomb products extend beyond their well-known activity as transcriptional regulators to include genome duplication processes. Polycomb activities during DNA replication and DNA damage repair are unclear, particularly without induced replicative stress.We have used a cellularmodel of conditionally inactive polycomb E3 ligases (RING1A and RING1B), which monoubiquitylate lysine 119 of histone H2A (H2AK119Ub), to examine DNA replication in unperturbed cells. We identify slow elongation and fork stalling during DNA replication that is associated with the accumulation of mid and late S-phase cells. Signs of replicative stress and colocalisation of double-strand breaks with chromocenters, the sites of coalesced pericentromeric heterocromatic (PCH) domains, were enriched in cells at mid S-phase, the stage at which PCH is replicated. Altered replication was rescued by targeted monoubiquitylation of PCH through methyl- CpG binding domain protein 1. The acute senescence associated with the depletion of RING1 proteins, which is mediated by p21 (also known as CDKN1A) upregulation, could be uncoupled from a response to DNA damage. These findings link cell proliferation and the polycomb proteins RING1A and RING1B to S-phase progression through a specific function in PCH replication. |
Agencias financiadoras | Ministerio de Economía y Competitividad (MINECO). España |
Identificador del proyecto | info:eu-repo/grantAgreement/MINECO/BFU2010-18146
info:eu-repo/grantAgreement/MINECO/SAF2013-47997 info:eu-repo/grantAgreement/MINECO/BFU2013-42918-P |
Cita | Bravo, M., Nicolini, F., Starowicz, K., Barroso, S., Calés, C., Aguilera López, A. y Vidal, M. (2015). Polycomb RING1A- and RING1B-dependent histone H2A monoubiquitylation at pericentromeric regions promotes S-phase progression. Journal of Cell Science, 128 (19), 3660-3671. |
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Polycomb RING1A.pdf | 1.878Mb | [PDF] | Ver/ | |