Artículo
Na+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortex
Autor/es | Horra Padilla, Carmen de la
Cano Rodríguez, María Mercedes Peral Rubio, María José García Delgado, Marta Durán Martínez, Juan Manuel Calonge Castrillo, María Luisa Ilundáin Larrañeta, María Anunciación Ana |
Departamento | Universidad de Sevilla. Departamento de Fisiología |
Fecha de publicación | 2001 |
Fecha de depósito | 2020-04-24 |
Publicado en |
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Resumen | The presence of a Na+/D-mannose cotransport activity in brush-border membrane vesicles (BBMV), isolated from either rat small intestine or rat kidney cortex, is examined. In the presence of an electrochemical Na+ gradient, ... The presence of a Na+/D-mannose cotransport activity in brush-border membrane vesicles (BBMV), isolated from either rat small intestine or rat kidney cortex, is examined. In the presence of an electrochemical Na+ gradient, but not in its absence, D-mannose was transiently accumulated by the BBMV. D-Mannose uptake into the BBMV was energized by both the electrical membrane potential and the Na+ chemical gradient. D-Mannose transport vs. external D-mannose concentration can be described by an equation that represents a superposition of a saturable component and another component that cannot be saturated up to 50 μM D-mannose. D-Mannose uptake was inhibited by D-mannose ≫ D-glucose > phlorizin, whereas for α-methyl glucopyranoside the order was D-glucose = phlorizin ≫ D-mannose. The initial rate of D-mannose uptake increased as the extravesicular Na+ concentration increased, with a Hill coefficient of 1, suggesting that the Na+ :D-mannose cotransport stoichiometry is 1:1. It is concluded that both rat intestinal and renal apical membrane have a concentrative, saturable, electrogenic and Na+-dependent D-mannose transport mechanism, which is different from SGLT1. |
Identificador del proyecto | PM99-0121 |
Cita | Horra Padilla, C.d.l., Cano Rodríguez, M.M., Peral Rubio, M.J., García Delgado, M., Durán Martínez, J.M., Calonge Castrillo, M.L. y Ilundáin Larrañeta, M.A.A. (2001). Na+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortex. Biochimica et Biophysica Acta - Biomembranes, 1512 (2), 225-230. |
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