dc.creator | Ramírez, Marco Antonio | es |
dc.creator | Morales, Jorge | es |
dc.creator | Cornejo, Marcelo | es |
dc.creator | Blanco, Elias H. | es |
dc.creator | Mancilla-Sierpe, Edgardo | es |
dc.creator | Toledo, Fernando | es |
dc.creator | Beltrán, Ana Rosa | es |
dc.creator | Sobrevia Luarte, Luis | es |
dc.date.accessioned | 2019-06-10T14:38:37Z | |
dc.date.available | 2019-06-10T14:38:37Z | |
dc.date.issued | 2018 | |
dc.identifier.citation | Ramírez, M.A., Morales, J., Cornejo, M., Blanco, E.H., Mancilla-Sierpe, E., Toledo, F.,...,Sobrevia Luarte, L. (2018). Intracellular acidification reduces L-arginine transport via system y + L but not via system y + /CATs and nitric oxide synthase activity in human umbilical vein endothelial cells. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1864 (4), 1192-1202. | |
dc.identifier.issn | 0925-4439 | es |
dc.identifier.uri | https://hdl.handle.net/11441/87295 | |
dc.description.abstract | L-Arginine is taken up via the cationic amino acid transporters (system y + /CATs) and system y + L in human umbilical vein endothelial cells (HUVECs). L-Arginine is the substrate for endothelial NO synthase (eNOS) which is activated by intracellular alkalization, but nothing is known regarding modulation of system y + /CATs and system y + L activity, and eNOS activity by the pHi in HUVECs. We studied whether an acidic pHi modulates L-arginine transport and eNOS activity in HUVECs. Cells loaded with a pH-sensitive probe were subjected to 0.1–20 mmol/L NH 4 Cl pulse assay to generate pHi 7.13–6.55. Before pHi started to recover, L-arginine transport (0–20 or 0–1000 μmol/L, 10 s, 37 °C) in the absence or presence of 200 μmol/L N-ethylmaleimide (NEM) (system y + /CATs inhibitor) or 2 mmol/L L-leucine (systemy + L substrate) was measured. Protein abundance for eNOS and serine 1177 or threonine 495 phosphorylated eNOS was determined. The results show that intracellular acidification reduced system y + L but not system y + /CATs mediated L-arginine maximal transport capacity due to reduced maximal velocity. Acidic pHi reduced NO synthesis and eNOS serine 1177 phosphorylation. Thus, system y + L activity is downregulated by an acidic pHi, a phenomenon that may result in reduced NO synthesis in HUVECs. | es |
dc.description.sponsorship | Fondo Nacional de Desarrollo Científico y Tecnológico 1150377 | es |
dc.description.sponsorship | Universidad de Antofagasta 5309 , 5313 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Elsevier | es |
dc.relation.ispartof | Biochimica et Biophysica Acta - Molecular Basis of Disease, 1864 (4), 1192-1202. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Endothelium | es |
dc.subject | Intracellular pH | es |
dc.subject | L-Arginine transport | es |
dc.subject | System y + | es |
dc.subject | System y + L | es |
dc.title | Intracellular acidification reduces L-arginine transport via system y + L but not via system y + /CATs and nitric oxide synthase activity in human umbilical vein endothelial cells | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Fisiología | es |
dc.relation.projectID | 1150377 | es |
dc.relation.projectID | 5309 | es |
dc.relation.projectID | 5313 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.bbadis.2018.01.032 | es |
dc.identifier.doi | 10.1016/j.bbadis.2018.01.032 | es |
idus.format.extent | 11 p. | es |
dc.journaltitle | Biochimica et Biophysica Acta - Molecular Basis of Disease | es |
dc.publication.volumen | 1864 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 1192 | es |
dc.publication.endPage | 1202 | es |