Article
Glycosylated nanostructures in sublingual immunotherapy induce long-lasting tolerance in LTP allergy mouse model
Author/s | Rodríguez, María J.
Ramos Soriano, Francisco Javier Perkins, James R. Mascaraque González, Ainhoa Torres, María J. Gómez, Francisca Díaz Perales, Araceli Rojo, Javier Mayorga, Cristobalina |
Department | Instituto de Investigaciones Químicas (IIQ) – CIC Cartuja |
Publication Date | 2019-02-11 |
Deposit Date | 2019-05-28 |
Published in |
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Abstract | An effective specific immunotherapy should contain elements to generate specific recognition (T-cell peptides) and to modulate the immunological response towards a Th1/Treg pattern by enhancing dendritic cells (DCs). We ... An effective specific immunotherapy should contain elements to generate specific recognition (T-cell peptides) and to modulate the immunological response towards a Th1/Treg pattern by enhancing dendritic cells (DCs). We propose a novel sublingual immunotherapy for peach allergy, using systems, that combine Prup3-T-cell peptides with mannose dendrons (D1ManPrup3 and D4ManPrup3). Peach anaphylactic mice were treated 1, 2 and 5 nM concentrations. Tolerance was assessed one/five weeks after finishing treatment by determining in vivo/in vitro parameters after challenge with Prup3. Only mice receiving D1ManPrup3 at 2 nM were protected from anaphylaxis (no temperature changes, decrease in Prup3-sIgE and -sIgG1 antibody levels, and secreting cells) compared to PBS-treated mice. Moreover, an increase of Treg-cells and regulatory cytokines (IL-10+/IFN-γ+) in CD4+-T-cells and DCs were found. These changes were maintained at least five weeks after stopping treatment. D1ManPrup3 is an effective new approach of immunotherapy inducing protection from anaphylaxis which persists after finishing treatment. |
Project ID. | PI12/02481
PI15/00559 PI18/00288 RD16/0006/0001 RD16/0006/0003 RD16/0006/0011 CD14/00242 CTS-7433 C-0044-2012 SAS2013 CTQ2014-52328-P CTQ2017-86265-P AGL2014-52395-C2-2 |
Citation | Rodríguez, M.J., Ramos Soriano, F.J., Perkins, J.R., Mascaraque González, A., Torres, M.J., Gómez, F.,...,Mayorga, C. (2019). Glycosylated nanostructures in sublingual immunotherapy induce long-lasting tolerance in LTP allergy mouse model. Scientific Reports, 9 (1), 4043-1-4043-9. |
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