Article
Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder
Author/s | Hmadcha, Abdelkrim
Aguilera, Yolanda Lozano Arana, María Dolores Mellado, Nuria Sánchez, Javier Moya, Cristina Sánchez Palazón, Luis Palacios Calvo, José Antiñolo Gil, Guillermo Soria Escoms, Bernat |
Department | Universidad de Sevilla. Departamento de Cirugía |
Publication Date | 2016 |
Deposit Date | 2019-01-28 |
Published in |
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Abstract | From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis
(PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were
successfully ... From 106 human blastocyts donate for research after in vitro fertilization (IVF) and preimplantation genetic diagnosis (PGD) for monogenetic disorder, 3 human embryonic stem cells (hESCs) HVR1, HVR2 and HVR3 were successfully derived. HVR1 was assumed to be genetically normal, HVR2 carrying Becker muscular dystrophy and HVR3 Hemophilia B. Despite the translocation t(9;15)(q34.3;q14) detected in HVR2, all the 3 cell lines were characterised in vitro and in vivo as normal hESCs lines and were registered in the Spanish Stem Cell Bank. |
Funding agencies | Junta de Andalucía Instituto de Salud Carlos III |
Project ID. | TCMR0021/06
PI246-2008 RD12/0019/0028 RD012/0036/0017 PI10/00964 PI11/02923 PI14/01015 |
Citation | Hmadcha, A., Aguilera, Y., Lozano Arana, M.D., Mellado, N., Sánchez, J., Moya, C.,...,Soria Escoms, B. (2016). Derivation of HVR1, HVR2 and HVR3 human embryonic stem cell lines from IVF embryos after preimplantation genetic diagnosis (PGD) for monogenic disorder. Stem Cell Research, 16, 635-639. |
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