Article
Zim17/Tim15 links mitochondrial iron–sulfur cluster biosynthesis to nuclear genome stability
Author/s | Díaz de la Loza, María del Carmen
Gallardo Ortega, Mercedes García Rubio, María Luisa Izquierdo, Alicia Herrero, Enrique Aguilera López, Andrés Wellinger, Ralf Erik |
Department | Universidad de Sevilla. Departamento de Genética |
Publication Date | 2011 |
Deposit Date | 2018-03-21 |
Published in |
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Abstract | Genomic instability is related to a wide-range of human diseases. Here, we show that mitochondrial iron–sulfur cluster biosynthesis is important for the maintenance of nuclear genome stability in Saccharomyces cerevisiae. ... Genomic instability is related to a wide-range of human diseases. Here, we show that mitochondrial iron–sulfur cluster biosynthesis is important for the maintenance of nuclear genome stability in Saccharomyces cerevisiae. Cells lacking the mitochondrial chaperone Zim17 (Tim15/Hep1), a component of the iron–sulfur biosynthesis machinery, have limited respiration activity, mimic the metabolic response to iron starvation and suffer a dramatic increase in nuclear genome recombination. Increased oxidative damage or deficient DNA repair do not account for the observed genomic hyperrecombination. Impaired cell-cycle progression and genetic interactions of ZIM17 with components of the RFC-like complex involved in mitotic checkpoints indicate that replicative stress causes hyperrecombination in zim17Δ mutants. Furthermore, nuclear accumulation of pre-ribosomal particles in zim17Δ mutants reinforces the importance of iron–sulfur clusters in normal ribosome biosynthesis. We propose that compromised ribosome biosynthesis and cell-cycle progression are interconnected, together contributing to replicative stress and nuclear genome instability in zim17Δ mutants. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Project ID. | BIO2003-07172
BIO2006-08051 CSD2007-0015 P08-CTS-04297 |
Citation | Díaz de la Loza, M.d.C., Gallardo Ortega, M., García Rubio, M.L., Izquierdo, A., Herrero, E., Aguilera López, A. y Wellinger, R.E. (2011). Zim17/Tim15 links mitochondrial iron–sulfur cluster biosynthesis to nuclear genome stability. Nucleic Acids Research, 39 (14), 6002-6015. |
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