Artículo
FANCD2 facilitates replication through common fragile sites
Autor/es | Madireddy, Advaitha
Aguilera López, Andrés Herrera Moyano, Emilia García Rubio, María Luisa Owen, Nichole Olson, Susan |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2016 |
Fecha de depósito | 2017-12-13 |
Publicado en |
|
Resumen | Common fragile sites (CFSs) are genomic regions that are unstable under conditions of replicative stress. Although the characteristics of CFSs that render them vulnerable to stress are mainly associated with replication, ... Common fragile sites (CFSs) are genomic regions that are unstable under conditions of replicative stress. Although the characteristics of CFSs that render them vulnerable to stress are mainly associated with replication, the cellular pathways that protect CFSs during replication remain unclear. Here, we identify and describe a role for FANCD2 as a trans-acting facilitator of CFS replication, in the absence of exogenous replicative stress. In the absence of FANCD2, replication forks stall within the AT-rich fragility core of CFS leading to dormant origin activation. Furthermore, FANCD2 deficiency is associated with DNA:RNA hybrid formation at CFS-FRA16D and inhibition of DNA:RNA hybrid formation suppresses replication perturbation. In addition, we also found that FANCD2 reduces the number of potential sites of replication initiation. Our data demonstrate that FANCD2 protein is required to ensure efficient CFS replication and provide mechanistic insight into how FANCD2 regulates CFS stability. |
Cita | Madireddy, A., Aguilera López, A., Herrera Moyano, E., García Rubio, M.L., Owen, . y Olson, S. (2016). FANCD2 facilitates replication through common fragile sites. Molecular Cell, 64 (2), 388-404. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
pub14nihms821934.pdf | 4.099Mb | [PDF] | Ver/ | |