Artículo
Yeast Sen1 helicase protects the genome from transcription-associated instability
Autor/es | Mischo, Hannah E.
Gómez González, Belén Grzechnik, Pawel García Rondón, Ana Wei, Wu Steinmetz, Lars Aguilera López, Andrés Proudfoot, Nick J. |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2011-01-06 |
Fecha de depósito | 2017-11-22 |
Publicado en |
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Resumen | Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses ... Sen1 of S. cerevisiae is a known component of the NRD complex implicated in transcription termination of nonpolyadenylated as well as some polyadenylated RNA polymerase II transcripts. We now show that Sen1 helicase possesses a wider function by restricting the occurrence of RNA:DNA hybrids that may naturally form during transcription, when nascent RNA hybridizes to DNA prior to its packaging into RNA protein complexes. These hybrids displace the nontranscribed strand and create R loop structures. Loss of Sen1 results in transient R loop accumulation and so elicits transcription-associated recombination. SEN1 genetically interacts with DNA repair genes, suggesting that R loop resolution requires proteins involved in homologous recombination. Based on these findings, we propose that R loop formation is a frequent event during transcription and a key function of Sen1 is to prevent their accumulation and associated genome instability. |
Agencias financiadoras | Ministerio de Ciencia e Innovación (MICIN). España Junta de Andalucía |
Identificador del proyecto | BFU2006-05260
CSD2007-0015 BIO102 CV2549 |
Cita | Mischo, H.E., Gómez González, B., Grzechnik, P., García Rondón, A., Wei, W., Steinmetz, L.,...,Proudfoot, N.J. (2011). Yeast Sen1 helicase protects the genome from transcription-associated instability. Molecular Cell, 41 (1), 21-32. |
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Yeast Sen1.pdf | 1.193Mb | [PDF] | Ver/ | |