Article
Temperature- and salinity-decoupled overproduction of hydroxyectoine by chromohalobacter salexigens
Author/s | Nieto Gutiérrez, Joaquín José
![]() ![]() ![]() ![]() ![]() ![]() ![]() Rodríguez Moya, Javier Argandoña Bertrán, Montserrat ![]() ![]() ![]() ![]() ![]() ![]() ![]() Iglesias Guerra, Fernando ![]() ![]() ![]() ![]() ![]() ![]() Vargas Macías, Carmen ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
Department | Universidad de Sevilla. Departamento de Microbiología y Parasitología Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica |
Publication Date | 2012 |
Deposit Date | 2016-03-09 |
Published in |
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Abstract | Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a ... Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hy- droxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines. |
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