Artículo
Light-dependent functions of the Fusarium fujikuroi CryD DASH cryptochrome in development and secondary metabolism
Autor/es | Ávalos Cordero, Francisco Javier
Castrillo Jiménez, Marta García Martínez, Jorge |
Departamento | Universidad de Sevilla. Departamento de Genética |
Fecha de publicación | 2013 |
Fecha de depósito | 2016-02-24 |
Publicado en |
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Resumen | DASH (Drosophila, Arabidopsis, Synechocystis, human) cryptochromes (cry-DASHs) constitute a subgroup of the photolyase cryptochrome family with diverse light-sensing roles, found in most taxonomical groups. The genome of ... DASH (Drosophila, Arabidopsis, Synechocystis, human) cryptochromes (cry-DASHs) constitute a subgroup of the photolyase cryptochrome family with diverse light-sensing roles, found in most taxonomical groups. The genome of Fusarium fujikuroi,a phytopathogenic fungus with a rich secondary metabolism, contains a gene encoding a putative cry-DASH, named CryD. The expression of the cryD gene is induced by light in the wild type, but not in mutants of the “white collar” gene wcoA. Targeted acryD mutants show light-dependent phenotypic alterations, including changes in morphology and pigmentation, which disap- pear upon reintroduction of a wild-type cryD allele. In addition to microconidia, the colonies of the acryD mutants produced under illumination and nitrogen starvation large septated spores called macroconidia, absent in wild-type colonies. The acryD mutants accumulated similar amounts of carotenoids to the control strain under constant illumination, but produced much larger amounts of bikaverin under nitrogen starvation, indicating a repressing role for CryD in this biosynthetic pathway. Addi- tionally, a moderate photoinduction of gibberellin production was exhibited by the wild type but not by the acryD mutants. The phenotypic alterations of the acryD mutants were only noticeable in the light, as expected from the low expression of cryD in the dark, but did not correlate with mRNA levels for structural genes of the bikaverin or gibberellin biosynthetic pathways, suggest- ing the participation of CryD in posttranscriptional regulatory mechanisms. This is the first report on the participation of a cry- DASH protein in the regulation of fungal secondary metabolism. |
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