dc.creator | González Aguilera, Cristina | |
dc.creator | Tous Rivera, Cristina | |
dc.creator | Gómez González, Belén | |
dc.creator | Huertas Sánchez, Pablo | |
dc.creator | Luna Varo, Rosa María | |
dc.creator | Aguilera López, Andrés | |
dc.date.accessioned | 2015-10-05T12:26:53Z | |
dc.date.available | 2015-10-05T12:26:53Z | |
dc.date.issued | 2008 | |
dc.identifier.issn | 1059-1524 | es |
dc.identifier.issn | 1939-4586 | es |
dc.identifier.uri | http://hdl.handle.net/11441/29169 | |
dc.description.abstract | The eukaryotic THO/TREX complex, involved in mRNP biogenesis, plays a key role in the maintenance of genome integrity in yeast. mRNA export factors such as Thp1-Sac3 also affect genome integrity, but their mutations have other phenotypes different from those of THO/TREX. Sus1 is a novel component of SAGA transcription factor that also associates with Thp1-Sac3, but little is known about its effect on genome instability and transcription. Here we show that Thp1, Sac3, and Sus1 form a functional unit with a role in mRNP biogenesis and maintenance of genome integrity that is independent of SAGA. Importantly, the effects of ribozyme-containing transcription units, RNase H, and the action of human activation-induced cytidine deaminase on transcription and genome instability are consistent with the possibility that R-loops are formed in Thp1-Sac3-Sus1-Cdc31 as in THO mutants. Our data reveal that Thp1-Sac3-Sus1-Cdc31, together with THO/TREX, define a specific pathway connecting transcription elongation with export via an RNA-dependent dynamic process that provides a feedback mechanism for the control of transcription and the preservation of genetic integrity of transcribed DNA regions. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | American Society for Cell Biology | es |
dc.relation.ispartof | Molecular Biology of the Cell, 19(10), 4310-4318 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Cell Cycle Proteins/metabolism | |
dc.subject | Genomic Instability | |
dc.subject | Nuclear Proteins/metabolism | |
dc.subject | RNA, Messenger/metabolism | |
dc.subject | RNA-Binding Proteins/metabolism | |
dc.subject | Ribonucleoproteins/metabolism | |
dc.subject | Saccharomyces cerevisiae/physiology | |
dc.subject | Saccharomyces cerevisiae Proteins/metabolism | |
dc.subject | Transcription, Genetic | |
dc.subject | Calcium-Binding Proteins | es |
dc.subject | metabolism | es |
dc.title | The THP1-SAC3-SUS1-CDC31 complex works in transcription elongation-mRNA export preventing RNA-mediated genome instability | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1091/mbc.E08-04-0355 | es |
dc.identifier.doi | http://dx.doi.org/10.1091/mbc.E08-04-0355 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/29169 | |