Artículo
Implications of Two-component Systems EnvZ/OmpR and BaeS/BaeR in in Vitro Temocillin Resistance in Escherichia Coli
Autor/es | Pérez Palacios, Patricia
Rodríguez Ochoa, José Luis Velázquez Escudero, Ana Rodríguez-Baño, Jesús Rodríguez Martínez, José Manuel Pascual Hernández, Álvaro Docobo Pérez, Fernando |
Departamento | Universidad de Sevilla. Departamento de Microbiología Universidad de Sevilla. Departamento de Medicina |
Fecha de publicación | 2024 |
Fecha de depósito | 2024-10-18 |
Publicado en |
|
Resumen | Background: BaeS/BaeR is a two-component system of Escherichia coli that controls the expression of porins and efflux pumps. Its role in beta-lactam resistance is limited. Objectives: To study the role of baeS/baeR ... Background: BaeS/BaeR is a two-component system of Escherichia coli that controls the expression of porins and efflux pumps. Its role in beta-lactam resistance is limited. Objectives: To study the role of baeS/baeR two-component system in temocillin resistance in E. coli. Methods: E. coli strain BW25113 and single-gene deletion mutants related to two-component systems were collected from the KEIO collection. Double-gen deletion mutants were generated. Temocillin-resistant mutant frequencies were determined at 32 mg/L. E. coli BW25113 mutants were selected by selective pressure from serial passages. Biological costs were analysed by growth curves. Genomes of the generated mutants were sequenced. The expression level of the mdtA, mdtB, mdtC, acrD and tolC in the ΔbaeS mutant was determined by RT–PCR (with/without temocillin exposure). Results: The frequency of temocillin mutants ranged from 2.12 × 10−8 to 4.51 × 10−8 in single-porin mutants. No mutants were recovered from E. coli BW25113 (>10−9). Selection of temocillin-resistant variants by serial passage yielded mutants up to 128 mg/L. Mutations were found in the baeS gene. Temocillin MICs ranged from 4 to 32 mg/L (highest MICs for ΔbaeS and ΔompR). The efflux pumps mdtA, mdtB, mdtC and acrD pumps were overexpressed 3–10-fold in the presence of temocillin in ΔbaeS compared to control. Conclusions: Mutations in the sensor histidine kinase, baeS, may be involved in temocillin resistance through the expression of the efflux pumps mdtABC and acrD. In addition, the low mutation rate may be a good predictor of temocillin activity. |
Agencias financiadoras | Junta de Andalucía Sociedad Europea de Microbiología Clínica y Enfermedades Infecciosas (ECCMID) |
Identificador del proyecto | P20-00985’
RH-0136-2020 02561 |
Cita | Pérez Palacios, P., Rodríguez Ochoa, J.L., Velázquez Escudero, A., Rodríguez-Baño, J., Rodríguez Martínez, J.M., Pascual Hernández, Á. y Docobo Pérez, F. (2024). Implications of Two-component Systems EnvZ/OmpR and BaeS/BaeR in in Vitro Temocillin Resistance in Escherichia Coli. Journal of Antimicrobial Chemotherapy, 79 (3), 641-647. https://doi.org/10.1093/jac/dkae021. |
Ficheros | Tamaño | Formato | Ver | Descripción |
---|---|---|---|---|
Implications of two-component ... | 376.1Kb | [PDF] | Ver/ | |