Article
Analysis of sulfide signaling in rice highlights specific drought responses
Author/s | Zhang, Jing
Aroca Aguilar, Ángeles Hervás Morón, Manuel Navarro, José A. Moreno, Inmaculada Xie, Yanjie Romero, Luis C. Gotor Martínez, Cecilia |
Department | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular |
Publication Date | 2024-08-28 |
Deposit Date | 2024-09-26 |
Published in |
|
Abstract | Hydrogen sulfide regulates essential plant processes, including adaptation responses to stress situations, and the best characterized mechanism of action of sulfide consists of the post-translational modification of ... Hydrogen sulfide regulates essential plant processes, including adaptation responses to stress situations, and the best characterized mechanism of action of sulfide consists of the post-translational modification of persulfidation. In this study, we reveal the first persulfidation proteome described in rice including 3443 different persulfidated proteins that participate in a broad range of biological processes and metabolic pathways. In addition, comparative proteomics revealed specific proteins involved in sulfide signaling during drought responses. Several proteins are involved in the maintenance of cellular redox homeostasis, the tricarboxylic acid cycle and energy-related pathways, and ion transmembrane transport and cellular water homeostasis, with the aquaporin family showing the highest differential levels of persulfidation. We revealed that water transport activity is regulated by sulfide which correlates with an increasing level of persulfidation of aquaporins. Our findings emphasize the impact of persulfidation on total ATP levels, fatty acid composition, levels of reactive oxygen species, antioxidant enzymatic activities, and relative water content. Interestingly, the role of persulfidation in aquaporin transport activity as an adaptation response in rice differs from current knowledge of Arabidopsis, which highlights the distinct role of sulfide in improving rice tolerance to drought. |
Funding agencies | Ministerio de Ciencia e Innovación (MICIN). España Consejo Superior de Investigaciones Científicas (CSIC) Junta de Andalucía |
Project ID. | PID2019-109785GB-I00
PID2020-112645GB-I00 TED2021- 131443B-I00 PID2022-141885NB-I00 2023AEP077 P18-RT-3154 PROYEXCEL_00177 |
Citation | Zhang, J., Aroca Aguilar, Á., Hervás Morón, M., Navarro, J.A., Moreno, I., Xie, Y.,...,Gotor Martínez, C. (2024). Analysis of sulfide signaling in rice highlights specific drought responses. Journal of Experimental Botany, 75 (16), 5130-5145. https://doi.org/10.1093/jxb/erae249. |
Files | Size | Format | View | Description |
---|---|---|---|---|
Analysis of sulfide.pdf | 3.284Mb | [PDF] | View/ | Versión publicada |